The α subunit of RNA polymerase specifically inhibits expression of the porin genes ompF and ompC in vivo and in vitro in Escherichia coli

Valerie Bowrin, Renee Brissette, Kangla Tsung, Masayori Inouye

Research output: Contribution to journalArticle

10 Scopus citations


Overproduction of the α subunit of RNA polymerase in Escherichia coli resulted in inhibition of transcription of two osmoregulated porin genes, ompF and ompC, but not of constitutively expressed housekeeping genes. Overproduction of the σ subunit did not have any inhibitory effects. The specific inhibitory effect of the α subunit was also found to depend upon the OmpR protein, the transcriptional activator for ompF and ompC. These results are in general agreement with other biochemical and genetic evidence suggesting that α subunit is the subunit of RNA polymerase that directly interacts with certain transcriptional activators to initiate transcription.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalFEMS Microbiology Letters
Issue number1
StatePublished - Jan 1 1994


All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics


  • Escherichia coli
  • Inhibition
  • ompC
  • ompF
  • α Subunit

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