TY - JOUR
T1 - The σ70 subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter
AU - Nickels, Bryce E.
AU - Mukhopadhyay, Jayanta
AU - Garrity, Sean J.
AU - Ebright, Richard H.
AU - Hochschild, Ann
PY - 2004/6
Y1 - 2004/6
N2 - The σ70 subunit of RNA polymerase plays an essential role in transcription initiation. In addition, σ70 has a critical regulatory role during transcription elongation at the bacteriophage λ late promoter, σPR′. At this promoter, σ 70 mediates a pause in early elongation through contact with a DNA sequence element in the initially transcribed region that resembles a promoter -10 element. Here we provide evidence that σ70 also mediates a pause in early elongation at the lac promoter (plac). Like that at λPR′, the pause at plac is facilitated by a sequence element in the initially transcribed region that resembles a promoter -10 element. Using biophysical analysis, we demonstrate that the pause-inducing sequence element at plac stabilizes the interaction between σ 70 and the remainder of the transcription elongation complex. Bioinformatic analysis suggests that promoter-proximal σ 70-dependent pauses may play a role in the regulation of many bacterial promoters.
AB - The σ70 subunit of RNA polymerase plays an essential role in transcription initiation. In addition, σ70 has a critical regulatory role during transcription elongation at the bacteriophage λ late promoter, σPR′. At this promoter, σ 70 mediates a pause in early elongation through contact with a DNA sequence element in the initially transcribed region that resembles a promoter -10 element. Here we provide evidence that σ70 also mediates a pause in early elongation at the lac promoter (plac). Like that at λPR′, the pause at plac is facilitated by a sequence element in the initially transcribed region that resembles a promoter -10 element. Using biophysical analysis, we demonstrate that the pause-inducing sequence element at plac stabilizes the interaction between σ 70 and the remainder of the transcription elongation complex. Bioinformatic analysis suggests that promoter-proximal σ 70-dependent pauses may play a role in the regulation of many bacterial promoters.
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U2 - 10.1038/nsmb757
DO - 10.1038/nsmb757
M3 - Article
C2 - 15122345
AN - SCOPUS:2542498681
VL - 11
SP - 544
EP - 550
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
SN - 1545-9993
IS - 6
ER -