The σ70 subunit of RNA polymerase mediates a promoter-proximal pause at the lac promoter

Bryce E. Nickels, Jayanta Mukhopadhyay, Sean J. Garrity, Richard H. Ebright, Ann Hochschild

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

The σ70 subunit of RNA polymerase plays an essential role in transcription initiation. In addition, σ70 has a critical regulatory role during transcription elongation at the bacteriophage λ late promoter, σPR′. At this promoter, σ 70 mediates a pause in early elongation through contact with a DNA sequence element in the initially transcribed region that resembles a promoter -10 element. Here we provide evidence that σ70 also mediates a pause in early elongation at the lac promoter (plac). Like that at λPR′, the pause at plac is facilitated by a sequence element in the initially transcribed region that resembles a promoter -10 element. Using biophysical analysis, we demonstrate that the pause-inducing sequence element at plac stabilizes the interaction between σ 70 and the remainder of the transcription elongation complex. Bioinformatic analysis suggests that promoter-proximal σ 70-dependent pauses may play a role in the regulation of many bacterial promoters.

Original languageEnglish (US)
Pages (from-to)544-550
Number of pages7
JournalNature Structural and Molecular Biology
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2004

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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