The 3′UTR of HIC mRNA improves the production of recombinant proteins in Chinese hamster ovary cells

Jaron Liu, Sebastian C.Y. Ku, Jialing Lee, Tara M. Young, Tsafi Pe'ery, Michael B. Mathews, Sheng Hao Chao

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Positive transcription elongation factor b (P-TEFb) is an important transcriptional regulator which controls 70-80% of RNA polymerase II transcription. It has been reported that the human I-mfa (inhibitor of MyoD family a) domain-containing protein (HIC) interacts with P-TEFb and that expression of HIC cDNA stimulates P-TEFb-dependent transcription. Interestingly, our recent study shows that transcriptional stimulation by HIC is predominately due to the 3′ untranslated region (3′UTR) of HIC mRNA rather than its coding region. In this report, we investigate the effects of HIC 3′UTR on recombinant protein expression in mammalian cells. In transient transfections, overexpression of HIC 3′UTR stimulates transgene expression in several mammalian cell lines and significantly increases the production of human erythropoietin and interferon-γ in Chinese hamster ovary (CHO) cells. This is the first report that demonstrates the improvement of expression of biopharmaceutical proteins by overexpressing a non-coding 3′UTR in CHO cells.

Original languageEnglish (US)
Pages (from-to)152-155
Number of pages4
JournalJournal of Biotechnology
Volume139
Issue number2
DOIs
StatePublished - Jan 15 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Keywords

  • 3′UTR
  • Erythropoietin
  • Human I-mfa domain-containing protein
  • Interferon-γ
  • P-TEFb
  • RNA polymerase II

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