The actin gene of the glaucocystophyte Cyanophora paradoxa: Analysis of the coding region and introns, and an actin phylogeny of eukaryotes

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Abstract

We isolated the actin gene of the glaucocystophyte alga Cyanophora paradoxa and analyzed the coding region and its introns. Phylogenetic analyses of the actin coding region and the inferred protein sequence in data sets containing 47 other actin sequences show Cyanophora to be a member of the eukaryotic crown-group radiation in agreement with ribosomal DNA sequence analyses. Four of the five Cyanophora actin introns are relatively short (55-59 nt) and occupy novel positions in a catalogue of actin introns containing 56 distinct sites. The fifth intron has a length of 171 nt and occurs also in actin genes from green algae and the crustacean Artemia.

Original languageEnglish (US)
Pages (from-to)439-446
Number of pages8
JournalCurrent Genetics
Volume31
Issue number5
DOIs
StatePublished - May 1 1997

Fingerprint

Cyanophora
Phylogeny
Eukaryota
Introns
Actins
Genes
Artemia
Chlorophyta
Ribosomal DNA
Crowns
DNA Sequence Analysis
Open Reading Frames
Radiation

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Actin
  • Cyanophora paradoxa
  • Introns
  • Phylogeny

Cite this

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abstract = "We isolated the actin gene of the glaucocystophyte alga Cyanophora paradoxa and analyzed the coding region and its introns. Phylogenetic analyses of the actin coding region and the inferred protein sequence in data sets containing 47 other actin sequences show Cyanophora to be a member of the eukaryotic crown-group radiation in agreement with ribosomal DNA sequence analyses. Four of the five Cyanophora actin introns are relatively short (55-59 nt) and occupy novel positions in a catalogue of actin introns containing 56 distinct sites. The fifth intron has a length of 171 nt and occurs also in actin genes from green algae and the crustacean Artemia.",
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T1 - The actin gene of the glaucocystophyte Cyanophora paradoxa

T2 - Analysis of the coding region and introns, and an actin phylogeny of eukaryotes

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AU - Weber, Klaus

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AB - We isolated the actin gene of the glaucocystophyte alga Cyanophora paradoxa and analyzed the coding region and its introns. Phylogenetic analyses of the actin coding region and the inferred protein sequence in data sets containing 47 other actin sequences show Cyanophora to be a member of the eukaryotic crown-group radiation in agreement with ribosomal DNA sequence analyses. Four of the five Cyanophora actin introns are relatively short (55-59 nt) and occupy novel positions in a catalogue of actin introns containing 56 distinct sites. The fifth intron has a length of 171 nt and occurs also in actin genes from green algae and the crustacean Artemia.

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