The affect of cadmium on sulfate assimilation enzymes in Brassica juncea

Sangman Lee, Thomas Leustek

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

The expression of mRNA and enzyme activity for the sulfur assimilation enzymes ATP sulfurylase (AS) and APS reductase (AR) was measured over 24 h in Brassica juncea exposed to 50 μM CdSO4. AS mRNA increased ≃3-fold only in roots and there was no change in enzyme activity. AR mRNA level was unaffected in leaves but enzyme activity declined by ≃7-fold. In roots AR mRNA and enzyme increased asynchronously; mRNA increased ≃5-fold after 6 h while enzyme activity increased ≃5-fold in 24 h. In plants exposed- for 3 h to Cd and then transferred to Cd-free medium AR activity increased ≃5-fold more rapidly than in plants that were continuously exposed. Feeding of cysteine or glutathione abolished the Cd-induced increases in mRNA and enzyme activity. In plants that were exposed to varying Cd doses up to 200 μM the level of AR mRNA increased linearly with the dose but the activity lagged behind the mRNA and was inhibited above 50 μM. These results indicate that the lag in-enzyme expression is likely due to an effect of Cd. However, not all heavy metals had the same effect. When treated with Zn, Pb, Cu, and Hg AR mRNA and enzyme activity increased synchronously.

Original languageEnglish (US)
Pages (from-to)201-207
Number of pages7
JournalPlant Science
Volume141
Issue number2
DOIs
StatePublished - Feb 22 1999

All Science Journal Classification (ASJC) codes

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Keywords

  • Brassica juncea
  • Cadmium
  • Sulphate assimilation enzymes

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