The bioluminescent imaging of spontaneously occurring tumors in immunocompetent ODD-luciferase bearing transgenic mice

Scott J. Goldman, Shengkan Jin

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The imaging of spontaneously occurring tumors in mice poses many technical and logistical problems. Recently a mouse model was generated in which a chimeric protein consisting of HIF-1α oxygen-dependent degradation domain (ODD) fused to luciferase was ubiquitously expressed in all tissues. Hypoxic stress leads to the accumulation of ODD-luciferase in the tissues of this mouse model which can be identified by noninvasive bioluminescence measurement. Crossing this transgenic mouse with tumorigenic mice yields solid tumors with hypoxic cores that may be successfully imaged and characterized using the technique described herein.

Original languageEnglish (US)
Title of host publicationBioluminescent Imaging
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages129-143
Number of pages15
ISBN (Print)9781627037174
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1098
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Bioluminescence
  • HIF
  • Hypoxia
  • In-vivo
  • ODD-luciferase
  • Tumor

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