The cellular repressor of E1A-stimulated genes induces differentiation and migration of human vascular smooth muscle cells

Ya Ling Han, Ye Hu, Hai Wei Liu, Jian Kang, Cheng Hui Yan, Shao Hua Li

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

In order to study the effect of the cellular repressor of E1A-stimulated genes (CREG) on differentiation and migration of human vascular smooth muscle cells (VSMCs)-HITASY, the full length human sense and antisense-CREG cDNA retroviral vectors, pLNCX2(+)/CREG and pLXSN(-)/CREG, were constructed. Western blot and immunoflourescence analysis showed that the expression of CREG and SMα -actin increased in HITASY after infection with pLNCX2(+) /CREG. The migration of HITASY infected with pLNCX 2(+)/CREG obviously enhanced compared with that of normal HITASY and pLXSN(-)/CREG cells showed by scrape-wounding and time-lapse analysis. Moreover, CREG over-expression increased the secretion of MMPs in HITASY tested by Western blot. Gelatin SDS-PAGE zymography analysis revealed that the activities of MMPs also increased in HITASY infected with pLNCX2(+)/CREG. On the other hand, the opposite effects were observed when CREG expression decreased by using antisense pLXSN(-)/CREG. These results suggest that CREG may be able to induce the VSMCs differetiation and promote the VSMCs migration in the meantime.

Original languageEnglish (US)
Pages (from-to)517-522
Number of pages6
JournalProgress in Biochemistry and Biophysics
Volume32
Issue number6
StatePublished - Jun 2005

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Keywords

  • Adenoviral protein E1A
  • Differentiation
  • Migration
  • Repressor
  • Retrovirus vector
  • Smooth muscle

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