The clustering of axonal sodium channels during development of the peripheral nervous system

Ian Vabnick, Sanja D. Novaković, S. Rock Levinson, Melitta Schachner, Peter Shrager

Research output: Contribution to journalArticlepeer-review

116 Scopus citations

Abstract

The distribution of Na+ channels in rat peripheral nerve was measured during development by using immunofluorescence. Small segments of sciatic nerve from postnatal day 0-13 (P0-P13) pups were labeled with an antibody raised against a well conserved region of the vertebrate Na+ channel. At day P0 axons contained almost no Na+ channel aggregates. The number of clusters increased dramatically throughout the first week. In almost all cases Na+ channels clustered in the vicinity of Schwann cell processes. At least four classes of aggregates were noted. Clusters formed singly at Schwann cell edges, in pairs or in broad regions between neighboring Schwann cells, and in more focal zones at presumptive nodes. Almost all Na+ channel aggregates had reached the latter stage by the end of the first week. Histograms plotting the frequency of occurrence of each cluster type suggested a sequence of events in node formation involving the initiation of channel aggregation by Schwann cell processes. The requirement for Schwann cells during sodium channel clustering was tested by blocking proliferation of these cells with the antimitotic agent mitomycin C. Na+ channel clustering was sharply reduced, whereas node formation was normal at a distal site along the same nerve. Immunocytochemical detection of myelin-associated glycoprotein (MAG) indicated that Schwann cells must begin to ensheathe axons before inducing Na+ channel clustering.

Original languageEnglish (US)
Pages (from-to)4914-4922
Number of pages9
JournalJournal of Neuroscience
Volume16
Issue number16
DOIs
StatePublished - Aug 15 1996

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Keywords

  • Schwann cell
  • axon
  • development
  • glia
  • myelin
  • sodium channel

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