The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencing

Richard C. Gardner, Alan J. Howarth, Peter Hahn, Marianne Brown-Luedi, Robert J. Shepherd, Joachim Messing

Research output: Contribution to journalArticlepeer-review

293 Scopus citations

Abstract

We have determined the complete primary structure (8031 base pairs) of an infectious clone of cauliflower mosaic virus strain CM1841. The sequence was obtained using the strategy of cloning shotgun restriction fragments in the sequencing vector M13mp7. Comparison of the CM1841 sequence with that published for another CaMV strain (Strasbourg) reveals 4.4% changes, mostly nucleotide substitutions with a few small insertions and deletions. The six open reading frames in the sequence of the Strasbourg isolate are also present in CM1841.

Original languageEnglish (US)
Pages (from-to)2871-2888
Number of pages18
JournalNucleic acids research
Volume9
Issue number12
DOIs
StatePublished - Jun 25 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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