The Connexin40A96S mutation from a patient with atrial fibrillation causes decreased atrial conduction velocities and sustained episodes of induced atrial fibrillation in mice

Indra Lübkemeier, René Andrié, Lars Lickfett, Felicitas Bosen, Florian Stöckigt, Radoslaw Dobrowolski, Astrid M. Draffehn, Julien Fregeac, Joachim L. Schultze, Feliksas F. Bukauskas, Jan Wilko Schrickel, Klaus Willecke

Research output: Contribution to journalArticle

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Abstract

Atrial fibrillation (AF) is the most common type of cardiac arrhythmia and a major cause of stroke. In the mammalian heart the gap junction proteins connexin40 (Cx40) and connexin43 (Cx43) are strongly expressed in the atrial myocardium mediating effective propagation of electrical impulses. Different heterozygous mutations in the coding region for Cx40 were identified in patients with AF. We have generated transgenic Cx40A96S mice harboring one of these mutations, the loss-of-function Cx40A96S mutation, as a model for atrial fibrillation. Cx40A96S mice were characterized by immunochemical and electrophysiological analyses. Significantly reduced atrial conduction velocities and strongly prolonged episodes of atrial fibrillation were found after induction in Cx40A96S mice. Analyses of the gating properties of Cx40A96S channels in cultured HeLa cells also revealed significantly lower junctional conductance and enhanced sensitivity voltage gating of Cx40A96S in comparison to Cx40 wild-type gap junctions. This is caused by reduced open probabilities of Cx40A96S gap junction channels, while single channel conductance remained the same. Similar to the corresponding patient, heterozygous Cx40A96S mice revealed normal expression levels and localization of the Cx40 protein. We conclude that heterozygous Cx40A96S mice exhibit prolonged episodes of induced atrial fibrillation and severely reduced atrial conduction velocities similar to the corresponding human patient.

Original languageEnglish (US)
Pages (from-to)19-32
Number of pages14
JournalJournal of Molecular and Cellular Cardiology
Volume65
DOIs
StatePublished - Dec 1 2013

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Atrial Fibrillation
Mutation
Gap Junctions
Connexin 43
Connexins
HeLa Cells
Transgenic Mice
Cardiac Arrhythmias
Cultured Cells
Myocardium
Stroke
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Lübkemeier, Indra ; Andrié, René ; Lickfett, Lars ; Bosen, Felicitas ; Stöckigt, Florian ; Dobrowolski, Radoslaw ; Draffehn, Astrid M. ; Fregeac, Julien ; Schultze, Joachim L. ; Bukauskas, Feliksas F. ; Schrickel, Jan Wilko ; Willecke, Klaus. / The Connexin40A96S mutation from a patient with atrial fibrillation causes decreased atrial conduction velocities and sustained episodes of induced atrial fibrillation in mice. In: Journal of Molecular and Cellular Cardiology. 2013 ; Vol. 65. pp. 19-32.
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abstract = "Atrial fibrillation (AF) is the most common type of cardiac arrhythmia and a major cause of stroke. In the mammalian heart the gap junction proteins connexin40 (Cx40) and connexin43 (Cx43) are strongly expressed in the atrial myocardium mediating effective propagation of electrical impulses. Different heterozygous mutations in the coding region for Cx40 were identified in patients with AF. We have generated transgenic Cx40A96S mice harboring one of these mutations, the loss-of-function Cx40A96S mutation, as a model for atrial fibrillation. Cx40A96S mice were characterized by immunochemical and electrophysiological analyses. Significantly reduced atrial conduction velocities and strongly prolonged episodes of atrial fibrillation were found after induction in Cx40A96S mice. Analyses of the gating properties of Cx40A96S channels in cultured HeLa cells also revealed significantly lower junctional conductance and enhanced sensitivity voltage gating of Cx40A96S in comparison to Cx40 wild-type gap junctions. This is caused by reduced open probabilities of Cx40A96S gap junction channels, while single channel conductance remained the same. Similar to the corresponding patient, heterozygous Cx40A96S mice revealed normal expression levels and localization of the Cx40 protein. We conclude that heterozygous Cx40A96S mice exhibit prolonged episodes of induced atrial fibrillation and severely reduced atrial conduction velocities similar to the corresponding human patient.",
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The Connexin40A96S mutation from a patient with atrial fibrillation causes decreased atrial conduction velocities and sustained episodes of induced atrial fibrillation in mice. / Lübkemeier, Indra; Andrié, René; Lickfett, Lars; Bosen, Felicitas; Stöckigt, Florian; Dobrowolski, Radoslaw; Draffehn, Astrid M.; Fregeac, Julien; Schultze, Joachim L.; Bukauskas, Feliksas F.; Schrickel, Jan Wilko; Willecke, Klaus.

In: Journal of Molecular and Cellular Cardiology, Vol. 65, 01.12.2013, p. 19-32.

Research output: Contribution to journalArticle

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AU - Lübkemeier, Indra

AU - Andrié, René

AU - Lickfett, Lars

AU - Bosen, Felicitas

AU - Stöckigt, Florian

AU - Dobrowolski, Radoslaw

AU - Draffehn, Astrid M.

AU - Fregeac, Julien

AU - Schultze, Joachim L.

AU - Bukauskas, Feliksas F.

AU - Schrickel, Jan Wilko

AU - Willecke, Klaus

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AB - Atrial fibrillation (AF) is the most common type of cardiac arrhythmia and a major cause of stroke. In the mammalian heart the gap junction proteins connexin40 (Cx40) and connexin43 (Cx43) are strongly expressed in the atrial myocardium mediating effective propagation of electrical impulses. Different heterozygous mutations in the coding region for Cx40 were identified in patients with AF. We have generated transgenic Cx40A96S mice harboring one of these mutations, the loss-of-function Cx40A96S mutation, as a model for atrial fibrillation. Cx40A96S mice were characterized by immunochemical and electrophysiological analyses. Significantly reduced atrial conduction velocities and strongly prolonged episodes of atrial fibrillation were found after induction in Cx40A96S mice. Analyses of the gating properties of Cx40A96S channels in cultured HeLa cells also revealed significantly lower junctional conductance and enhanced sensitivity voltage gating of Cx40A96S in comparison to Cx40 wild-type gap junctions. This is caused by reduced open probabilities of Cx40A96S gap junction channels, while single channel conductance remained the same. Similar to the corresponding patient, heterozygous Cx40A96S mice revealed normal expression levels and localization of the Cx40 protein. We conclude that heterozygous Cx40A96S mice exhibit prolonged episodes of induced atrial fibrillation and severely reduced atrial conduction velocities similar to the corresponding human patient.

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