The correlation of homocysteine-thiolactonase activity of the paraoxonase (PON1) protein with coronary heart disease status

T. B. Domagała, M. Łacinski, W. H. Trzeciak, B. Mackness, M. I. Mackness, Hieronim Jakubowski

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Homocysteine (Hcy)-thiolactonase (HTase) activity of the paraoxonase-1 (PON1) protein detoxifies Hcy-thiolactone in human blood and could thus delay the development of atherosclerosis. We investigated a hypothesis that HTase activity is associated with coronary heart disease. We studied HTase activities and PON1 genotypes in a group of 475 subjects, 42.5% of whom were healthy and 57.5% had coronary heart disease (CHD). We found that HTase activity was positively correlated with total cholesterol (r=0.254, P<0.0001), LDL cholesterol (0.149, P=0.016), ApoB (r=0.167, P=0.006), ApoA1 (0.140, P=0.023), and HDL cholesterol (0.184, P=0.002) in a group of CHD cases (n=270) but not in controls (n=202). Mean HTase activity was significantly higher in CHD cases than in controls (4.57 units vs. 3.30 units, P <10-5). The frequencies of the PON1-192 genotypes in CHD cases were similar to those in controls. HTase activity was not different between patients receiving statins and those not treated with statins. Multiple regression analysis shows that CHD status, PON1 genotype, and total cholesterol are determinants of HTase activity in humans. Our results suggest that HTase activity of the PON 1 protein is a predictor of CHD.

Original languageEnglish (US)
Pages (from-to)4-10
Number of pages7
JournalCellular and Molecular Biology
Volume52
Issue number5
DOIs
StatePublished - 2006

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • Age
  • Atherosclerosis
  • Genetic polymorphism
  • HDL
  • Homocysteine-thiolactonase
  • LDL
  • Paraoxonase
  • Statins
  • Total cholesterol

Fingerprint Dive into the research topics of 'The correlation of homocysteine-thiolactonase activity of the paraoxonase (PON1) protein with coronary heart disease status'. Together they form a unique fingerprint.

  • Cite this