The detection and subsequent volume optimization of biological nanocrystals

Joseph R. Luft; Jennifer R. Wolfley; Eleanor Cook Franks; Angela M. Lauricella; Ellen J. Gualtieri; Edward H. Snell; Rong Xiao; John K. Everett; Gaetano T. Montelione

doi:10.1063/1.4921199

The detection and subsequent volume optimization of biological nanocrystals

Joseph R. Luft, Jennifer R. Wolfley, Eleanor Cook Franks, Angela M. Lauricella, Ellen J. Gualtieri, Edward H. Snell, Rong Xiao, John K. Everett, Gaetano T. Montelione

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second-order nonlinear imaging of chiral crystals and ultraviolet two-photon excited fluorescence detection is shown to be applicable in a high-throughput manner to rapidly verify the presence of nanocrystals in crystallization screening conditions. It is noted that the nanocrystals are rarely seen without also producing microcrystals from other chemical conditions. A crystal volume optimization method is described and associated with a phase diagram for crystallization.

Original language	English (US)
Article number	041710
Journal	Structural Dynamics
Volume	2
Issue number	4
DOIs	https://doi.org/10.1063/1.4921199
State	Published - Jul 1 2015

All Science Journal Classification (ASJC) codes

Radiation
Instrumentation
Condensed Matter Physics
Spectroscopy

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title = "The detection and subsequent volume optimization of biological nanocrystals",

abstract = "Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second-order nonlinear imaging of chiral crystals and ultraviolet two-photon excited fluorescence detection is shown to be applicable in a high-throughput manner to rapidly verify the presence of nanocrystals in crystallization screening conditions. It is noted that the nanocrystals are rarely seen without also producing microcrystals from other chemical conditions. A crystal volume optimization method is described and associated with a phase diagram for crystallization.",

author = "Luft, {Joseph R.} and Wolfley, {Jennifer R.} and Franks, {Eleanor Cook} and Lauricella, {Angela M.} and Gualtieri, {Ellen J.} and Snell, {Edward H.} and Rong Xiao and Everett, {John K.} and Montelione, {Gaetano T.}",

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doi = "10.1063/1.4921199",

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AU - Luft, Joseph R.

AU - Wolfley, Jennifer R.

AU - Franks, Eleanor Cook

AU - Lauricella, Angela M.

AU - Gualtieri, Ellen J.

AU - Snell, Edward H.

AU - Xiao, Rong

AU - Everett, John K.

AU - Montelione, Gaetano T.

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second-order nonlinear imaging of chiral crystals and ultraviolet two-photon excited fluorescence detection is shown to be applicable in a high-throughput manner to rapidly verify the presence of nanocrystals in crystallization screening conditions. It is noted that the nanocrystals are rarely seen without also producing microcrystals from other chemical conditions. A crystal volume optimization method is described and associated with a phase diagram for crystallization.

AB - Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second-order nonlinear imaging of chiral crystals and ultraviolet two-photon excited fluorescence detection is shown to be applicable in a high-throughput manner to rapidly verify the presence of nanocrystals in crystallization screening conditions. It is noted that the nanocrystals are rarely seen without also producing microcrystals from other chemical conditions. A crystal volume optimization method is described and associated with a phase diagram for crystallization.

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The detection and subsequent volume optimization of biological nanocrystals

Abstract

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