The detection and subsequent volume optimization of biological nanocrystals

Joseph R. Luft, Jennifer R. Wolfley, Eleanor Cook Franks, Angela M. Lauricella, Ellen J. Gualtieri, Edward H. Snell, Rong Xiao, John K. Everett, Gaetano T. Montelione

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Identifying and then optimizing initial crystallization conditions is a prerequisite for macromolecular structure determination by crystallography. Improved technologies enable data collection on crystals that are difficult if not impossible to detect using visible imaging. The application of second-order nonlinear imaging of chiral crystals and ultraviolet two-photon excited fluorescence detection is shown to be applicable in a high-throughput manner to rapidly verify the presence of nanocrystals in crystallization screening conditions. It is noted that the nanocrystals are rarely seen without also producing microcrystals from other chemical conditions. A crystal volume optimization method is described and associated with a phase diagram for crystallization.

Original languageEnglish (US)
Article number041710
JournalStructural Dynamics
Issue number4
StatePublished - Jul 1 2015

All Science Journal Classification (ASJC) codes

  • Radiation
  • Instrumentation
  • Condensed Matter Physics
  • Spectroscopy


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