The detection of DNA adduct in human peripheral lymphocytes by bioluminescence method

Xianglin Tan, Shunqing Xu, Jian Chen, Hui Wang, Dongbing Liu

    Research output: Contribution to journalArticlepeer-review


    OBJECTIVE: To develop bioluminescence method to measure DNA adducts in human peripheral lymphocytes and study the factors that affect the level of DNA adduct, in order to use it in studying the relationship between water pollution and human DNA adduct levels. METHOD: The measurement of DNA adduct in peripheral lymphocytes was carried out in 234 normal persons, and the relationship between DNA adducts levels and age, smoking and eating habit etc. was analyzed. RESULTS: (1) The level of DNA adduct in males was higher than that in females, but without significance (P > 0.05); (2) The level of DNA adduct was significantly correlated with age, smoking, tea drinking, eating habit and alcohol drinking. Among them, age plus smoking were the most important factors affected the DNA adduct levels (P < 0.01). The level of DNA adduct increased with age. The more cigarette smoked, the higher was the level of DNA adduct. CONCLUSION: The level of DNA adduct is affected by many factors, so it needs further research to apply it in the biomonitoring and risk assessment of environment chemicals.

    Original languageEnglish (US)
    Pages (from-to)363-365
    Number of pages3
    JournalZhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
    Issue number6
    StatePublished - Nov 2002

    All Science Journal Classification (ASJC) codes

    • Medicine(all)


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