TY - JOUR
T1 - The Drosophila CLOCK protein undergoes daily rhythms in abundance, phosphorylation, and interactions with the PER-TIM complex
AU - Lee, Choogon
AU - Bae, Kiho
AU - Edery, Isaac
N1 - Funding Information:
This work was partially supported by an NIH grant (I. E.). We thank P. Hardin and H. Hao for providing the CaSpeR13.2per transformation vector and for help in generating transgenic flies bearing the 13.2per–HA10His construct.
PY - 1998/10
Y1 - 1998/10
N2 - We report the in vive characterization of the Drosophila CLOCK protein (dCLOCK), a transcription factor that is required for the expression of the circadian clock genes period (per) and timeless (tim). dCLOCK undergoes circadian fluctuations in abundance, is phosphorylated throughout a daily cycle, and interacts with PER, TIM, and/or the PER-TIM complex during the night but not during most of the day. Our results suggest that PER and TIM participate in transcriptional autoinhibition by physically interacting with dCLOCK or a dCLOCK-containing complex. Nevertheless, in the absence of PER or TIM, the levels of dCLOCK are constitutively low, indicating that PER and TIM also act as positive elements in the feedback loop by stimulating the production of dCLOCK.
AB - We report the in vive characterization of the Drosophila CLOCK protein (dCLOCK), a transcription factor that is required for the expression of the circadian clock genes period (per) and timeless (tim). dCLOCK undergoes circadian fluctuations in abundance, is phosphorylated throughout a daily cycle, and interacts with PER, TIM, and/or the PER-TIM complex during the night but not during most of the day. Our results suggest that PER and TIM participate in transcriptional autoinhibition by physically interacting with dCLOCK or a dCLOCK-containing complex. Nevertheless, in the absence of PER or TIM, the levels of dCLOCK are constitutively low, indicating that PER and TIM also act as positive elements in the feedback loop by stimulating the production of dCLOCK.
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U2 - 10.1016/S0896-6273(00)80601-7
DO - 10.1016/S0896-6273(00)80601-7
M3 - Article
C2 - 9808471
AN - SCOPUS:0032191902
VL - 21
SP - 857
EP - 867
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 4
ER -