The Drosophila homolog of NTF-2, the nuclear transport factor-2, is essential for immune response

Ananya Bhattacharya, Ruth Steward

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20 Scopus citations

Abstract

Nuclear transport factor-2 (NTF-2) functions in yeast and mammalian cell culture in targeting proteins into the nucleus. The Drosophila homolog, DNTF-2, is an essential component of the nuclear import machinery, since ntf mutants are lethal. Interestingly, hypomorphic alleles show specific phenotypes. Some are viable, but the number of omatidia in the eye is severely reduced. The immune response in the Drosophila larval fat body is also affected; the three NF-κB/Rel proteins Dorsal, Dif and Relish do not target to the nucleus after infection, and, consequently, the expression of the anti-microbial peptide genes drosomycin, attacin and drosocin is severely impaired. Hence, in spite of its general requirement in many developmental processes, DNTF-2 has a higher specific requirement in the development of the eye and in the immune response. We also found that DNTF-2 interacts directly with Mbo/DNup88, which does not contain phenylalanine-glycinerich repeats, but has been shown to function in the import of Rel proteins.

Original languageEnglish (US)
Pages (from-to)378-383
Number of pages6
JournalEMBO Reports
Volume3
Issue number4
DOIs
StatePublished - May 15 2002

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

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