TY - GEN
T1 - The effect of a simulated diabetic wound environment on keratinocyte migration
AU - Kosol, Wilai
AU - Faulknor, Renea
AU - Berthiaume, Francois
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015/6/2
Y1 - 2015/6/2
N2 - Chronic wounds, such as foot ulcers, are a common complication of diabetes. The environment in such wounds is a major factor in the failure of cellular processes that normally repair damaged skin to form a scar. It has been suggested that mesenchymal stromal cells (MSCs) may be used to promote the healing of chronic wounds. However, it is not known whether MSCs will be effective in the diabetic environment. This study aimed to set up an in vitro system to investigate the effect of MSCs on keratinocyte migration under a simulated diabetic wound environment, including hypoxia (1% O2 in the gas phase), infection (emulated by 1μg/ml bacterial endotoxin), and high glucose (25mM) medium. Because serum interferes with MSC-derived products, the effect of serum concentration of keratinocyte proliferation was investigated, and it was found that 1% v/v serum could be used with no adverse effects on keratinocyte proliferation. Keratinocytes were then grown to confluence, and a scratch was used to create a model wound. The effect of these diabetic conditions in conjunction with MSC-derived products on keratinocyte migration in the scratch assay is being measured.
AB - Chronic wounds, such as foot ulcers, are a common complication of diabetes. The environment in such wounds is a major factor in the failure of cellular processes that normally repair damaged skin to form a scar. It has been suggested that mesenchymal stromal cells (MSCs) may be used to promote the healing of chronic wounds. However, it is not known whether MSCs will be effective in the diabetic environment. This study aimed to set up an in vitro system to investigate the effect of MSCs on keratinocyte migration under a simulated diabetic wound environment, including hypoxia (1% O2 in the gas phase), infection (emulated by 1μg/ml bacterial endotoxin), and high glucose (25mM) medium. Because serum interferes with MSC-derived products, the effect of serum concentration of keratinocyte proliferation was investigated, and it was found that 1% v/v serum could be used with no adverse effects on keratinocyte proliferation. Keratinocytes were then grown to confluence, and a scratch was used to create a model wound. The effect of these diabetic conditions in conjunction with MSC-derived products on keratinocyte migration in the scratch assay is being measured.
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U2 - 10.1109/NEBEC.2015.7117041
DO - 10.1109/NEBEC.2015.7117041
M3 - Conference contribution
AN - SCOPUS:84941109776
T3 - 2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015
BT - 2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2015 41st Annual Northeast Biomedical Engineering Conference, NEBEC 2015
Y2 - 17 April 2015 through 19 April 2015
ER -