The effect of carbaryl and malathion in combination on γ-glutamyl transpeptidase and glutathione S-alkyltransferase activity in vitro

D. Waidron Lechner, M. S. Abdel-Rahman

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9 Scopus citations

Abstract

Exposure to pesticides is a serious environmental problem, since a realm of uncertainty exists concerning the toxic effects on man. Due to economical and more efficacious reasons, pesticides are being used in combination. The use of pesticides in combination, especially carbaryl and malathion, has become increasingly prevalent in recent years. Carbaryl and maiathion in combination are known to distribute to the rat liver and kidney to a greater extent than when exposure is limited to either pesticide alone. Since, carbaryl or malathion as individual pesticides are known to react with glutathione (GSH) during their metabolism and alterations in rat blood and liver GSH content after subacute exposure to this combination were noted in earlier work from this laboratory, it is important to examine the glutathione related parameters in the rat liver and kidney in vitro. Concentrations of 2 and 4 mM carbaryl and/or malathion were incubated with rat whole blood, liver and kidney homogenate and 10,000 × g supernatant to observe the effect of blood and liver glutathione (GSH) content, liver glutathione S-alkyl-transferase (GSAT) activity and liver and kidney γ-glutamyl transpeptidase (GGTP) levels. Liver homogenate GSH content significantly decreased with the increase in exposure time to carbaryl and/ or malathion, while blood GSH levels remained un-changed. Exposure of the liver GSAT enzyme with malathion and carbaryl/malathion in combination produced a two-fold increase in the enzyme's activity. Kidney GGTP activity was inhibited by carbaryl and/or malathion, while the activity in the liver was without change.

Original languageEnglish (US)
Pages (from-to)647-651
Number of pages5
JournalArchives of Environmental Contamination and Toxicology
Volume15
Issue number6
DOIs
StatePublished - Nov 1986

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

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