The effect of IL-13 and IL-13R130Q, a naturally occuring IL-13 polymorphism, on the gene expression of human airway smooth muscle cells

Farhat Syed, Reynold A. Panettieri, Omar Tliba, Chris Huang, Katherine Li, Michelle Bracht, Bernard Amegadzie, Don Griswold, Li Li, Yassine Amrani

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Background: Growing evidence shows that interleukin 13 (IL-13) may play an essential role in the development of airway inflammation and bronchial hyper-responsiveness (BHR), two defining features of asthma. Although the underlying mechanisms remain unknown, a number of reports have shown that IL-13 may exert its deleterious effects in asthma by directly acting on airway resident cells, including epithelial cells and airway smooth muscle cells. In this report, we hypothesize that IL-13 may participate in the pathogenesis of asthma by activating a set of "proasthmatic" genes in airway smooth muscle (ASM) cells. Methods: Microarray technology was used to study the modulation of gene expression of airway smooth muscle by IL-13 and IL-13R130Q. TaqMan™ Real Time PCR and flow cytometry was used to validate the gene array data. Results: IL-13 and the IL-13 polymorphism IL-13R130Q (Arg130Gln), recently associated with allergic asthma, seem to modulate the same set of genes, which encode many potentially interesting proteins including vascular cellular adhesion molecule (VCAM)-1, IL-13Rα2, Tenascin C and Histamine Receptor H1, that may be relevant for the pathogenesis of asthma. Conclusions: The data supports the hypothesis that gene modulation by IL-13 in ASM may be essential for the events leading to the development of allergic asthma.

Original languageEnglish (US)
Article number9
JournalRespiratory Research
Volume6
DOIs
StatePublished - Jan 20 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

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