The Effect of Swelling Ratio on the Coulter Underestimation of Hydrogel Microsphere Diameters

Michael Pellegrini, Abhimanyu Cherukupalli, Michael Medini, Ron Falkowski, Ronke Olabisi

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


It has been demonstrated that the diameters of porous particles are underestimated by Coulter measurements. This phenomenon has also been observed in hydrogel particles, but not characterized. Since the Coulter principle uses the displacement of electrolyte to determine particle size, electrolyte contained within the swelled hydrogel microparticles results in an underestimate of actual particle diameters. The increased use of hydrogel microspheres in biomedical applications has led to the increased application of the Coulter principle to evaluate the size distribution of microparticles. A relationship between the swelling ratio of the particles and their reported Coulter diameters will permit calculation of the actual diameters of these particles. Using polyethylene glycol diacrylate hydrogel microspheres, we determined a correction factor that relates the polymer swelling ratio and the reported Coulter diameters to their actual size.

Original languageEnglish (US)
Pages (from-to)1246-1250
Number of pages5
JournalTissue Engineering - Part C: Methods
Issue number12
StatePublished - Dec 1 2015

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

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