The effect of thrombospondin on oral squamous carcinoma cell invasion of collagen

Thomas N. Wang, Xiao hua Qian, Mark Granick, Mark P. Solomon, Vicki L. Rothman, George P. Tuszynski

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Thrombospondin (TSP), a cell matrix protein, and transforming growth factor ß (TGF-ß), a growth regulatory protein, play roles in tumor progression. The purpose of this study was to investigate the effects of TSP and TGF-ß on tumor cell invasion. Materials and methods: Tumor cell invasion assays were performed using a modified Boyden chamber apparatus with collagen-coated membranes. The KB oral carcinoma cell line was studied in serum-free media. Invasion was measured as the summation of the number of cells in five representative low-power fields (X 100) traversing the collagen barrier after a 3-hour incubation period. The effects of antibodies against TSP, TGF-ß and the cysteineserine-valine-threonine-cysteine-glycine (CSVTCG)-specific TSP receptor were also evaluated. Results: TSP caused a dose-dependent stimulation of tumor cell invasion. Antibodies against TSP, its CSVTCG-specific receptor, and TGF-ß inhibited TSP-promoted invasion by 50% to 71%. Conclusions: TSP and its CSVTCG-specific receptor promote KB cell invasion of collagen through the production and/or activation of TGF-ß.

Original languageEnglish (US)
Pages (from-to)502-505
Number of pages4
JournalThe American Journal of Surgery
Volume170
Issue number5
DOIs
StatePublished - Jan 1 1995

Fingerprint

Thrombospondins
Squamous Cell Carcinoma
Collagen
Transforming Growth Factors
Valine
Threonine
Glycine Receptors
Cysteine
Neoplasms
CD36 Antigens
KB Cells
Antibodies
Serum-Free Culture Media
Glycine
Proteins
Cell Count
Carcinoma
Cell Line
Membranes
Growth

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Wang, Thomas N. ; Qian, Xiao hua ; Granick, Mark ; Solomon, Mark P. ; Rothman, Vicki L. ; Tuszynski, George P. / The effect of thrombospondin on oral squamous carcinoma cell invasion of collagen. In: The American Journal of Surgery. 1995 ; Vol. 170, No. 5. pp. 502-505.
@article{4b5fbea0dcc5452e8dd557c8424e92d3,
title = "The effect of thrombospondin on oral squamous carcinoma cell invasion of collagen",
abstract = "Background: Thrombospondin (TSP), a cell matrix protein, and transforming growth factor {\ss} (TGF-{\ss}), a growth regulatory protein, play roles in tumor progression. The purpose of this study was to investigate the effects of TSP and TGF-{\ss} on tumor cell invasion. Materials and methods: Tumor cell invasion assays were performed using a modified Boyden chamber apparatus with collagen-coated membranes. The KB oral carcinoma cell line was studied in serum-free media. Invasion was measured as the summation of the number of cells in five representative low-power fields (X 100) traversing the collagen barrier after a 3-hour incubation period. The effects of antibodies against TSP, TGF-{\ss} and the cysteineserine-valine-threonine-cysteine-glycine (CSVTCG)-specific TSP receptor were also evaluated. Results: TSP caused a dose-dependent stimulation of tumor cell invasion. Antibodies against TSP, its CSVTCG-specific receptor, and TGF-{\ss} inhibited TSP-promoted invasion by 50{\%} to 71{\%}. Conclusions: TSP and its CSVTCG-specific receptor promote KB cell invasion of collagen through the production and/or activation of TGF-{\ss}.",
author = "Wang, {Thomas N.} and Qian, {Xiao hua} and Mark Granick and Solomon, {Mark P.} and Rothman, {Vicki L.} and Tuszynski, {George P.}",
year = "1995",
month = "1",
day = "1",
doi = "10.1016/S0002-9610(99)80340-7",
language = "English (US)",
volume = "170",
pages = "502--505",
journal = "American Journal of Surgery",
issn = "0002-9610",
publisher = "Elsevier Inc.",
number = "5",

}

The effect of thrombospondin on oral squamous carcinoma cell invasion of collagen. / Wang, Thomas N.; Qian, Xiao hua; Granick, Mark; Solomon, Mark P.; Rothman, Vicki L.; Tuszynski, George P.

In: The American Journal of Surgery, Vol. 170, No. 5, 01.01.1995, p. 502-505.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of thrombospondin on oral squamous carcinoma cell invasion of collagen

AU - Wang, Thomas N.

AU - Qian, Xiao hua

AU - Granick, Mark

AU - Solomon, Mark P.

AU - Rothman, Vicki L.

AU - Tuszynski, George P.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Background: Thrombospondin (TSP), a cell matrix protein, and transforming growth factor ß (TGF-ß), a growth regulatory protein, play roles in tumor progression. The purpose of this study was to investigate the effects of TSP and TGF-ß on tumor cell invasion. Materials and methods: Tumor cell invasion assays were performed using a modified Boyden chamber apparatus with collagen-coated membranes. The KB oral carcinoma cell line was studied in serum-free media. Invasion was measured as the summation of the number of cells in five representative low-power fields (X 100) traversing the collagen barrier after a 3-hour incubation period. The effects of antibodies against TSP, TGF-ß and the cysteineserine-valine-threonine-cysteine-glycine (CSVTCG)-specific TSP receptor were also evaluated. Results: TSP caused a dose-dependent stimulation of tumor cell invasion. Antibodies against TSP, its CSVTCG-specific receptor, and TGF-ß inhibited TSP-promoted invasion by 50% to 71%. Conclusions: TSP and its CSVTCG-specific receptor promote KB cell invasion of collagen through the production and/or activation of TGF-ß.

AB - Background: Thrombospondin (TSP), a cell matrix protein, and transforming growth factor ß (TGF-ß), a growth regulatory protein, play roles in tumor progression. The purpose of this study was to investigate the effects of TSP and TGF-ß on tumor cell invasion. Materials and methods: Tumor cell invasion assays were performed using a modified Boyden chamber apparatus with collagen-coated membranes. The KB oral carcinoma cell line was studied in serum-free media. Invasion was measured as the summation of the number of cells in five representative low-power fields (X 100) traversing the collagen barrier after a 3-hour incubation period. The effects of antibodies against TSP, TGF-ß and the cysteineserine-valine-threonine-cysteine-glycine (CSVTCG)-specific TSP receptor were also evaluated. Results: TSP caused a dose-dependent stimulation of tumor cell invasion. Antibodies against TSP, its CSVTCG-specific receptor, and TGF-ß inhibited TSP-promoted invasion by 50% to 71%. Conclusions: TSP and its CSVTCG-specific receptor promote KB cell invasion of collagen through the production and/or activation of TGF-ß.

UR - http://www.scopus.com/inward/record.url?scp=0028826079&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028826079&partnerID=8YFLogxK

U2 - 10.1016/S0002-9610(99)80340-7

DO - 10.1016/S0002-9610(99)80340-7

M3 - Article

C2 - 7485743

AN - SCOPUS:0028826079

VL - 170

SP - 502

EP - 505

JO - American Journal of Surgery

JF - American Journal of Surgery

SN - 0002-9610

IS - 5

ER -