The effect of thrombospondin on oral squamous carcinoma cell invasion of collagen

Thomas N. Wang, Xiao hua Qian, Mark S. Granick, Mark P. Solomon, Vicki L. Rothman, George P. Tuszynski

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Background: Thrombospondin (TSP), a cell matrix protein, and transforming growth factor ß (TGF-ß), a growth regulatory protein, play roles in tumor progression. The purpose of this study was to investigate the effects of TSP and TGF-ß on tumor cell invasion. Materials and methods: Tumor cell invasion assays were performed using a modified Boyden chamber apparatus with collagen-coated membranes. The KB oral carcinoma cell line was studied in serum-free media. Invasion was measured as the summation of the number of cells in five representative low-power fields (X 100) traversing the collagen barrier after a 3-hour incubation period. The effects of antibodies against TSP, TGF-ß and the cysteineserine-valine-threonine-cysteine-glycine (CSVTCG)-specific TSP receptor were also evaluated. Results: TSP caused a dose-dependent stimulation of tumor cell invasion. Antibodies against TSP, its CSVTCG-specific receptor, and TGF-ß inhibited TSP-promoted invasion by 50% to 71%. Conclusions: TSP and its CSVTCG-specific receptor promote KB cell invasion of collagen through the production and/or activation of TGF-ß.

Original languageEnglish (US)
Pages (from-to)502-505
Number of pages4
JournalThe American Journal of Surgery
Volume170
Issue number5
DOIs
StatePublished - Nov 1995

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All Science Journal Classification (ASJC) codes

  • Surgery

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