The effects of endothelin-1 on the PKA-dependent Cl- current in the heart.

A. F. James, L. H. Xie, M. Horie

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3 Scopus citations


The effects of endothelin-1 (ET-1) on whole-cell cardiac PKA-dependent Cl- currents (ICl) were investigated using patch clamp techniques. ET-1 inhibited the isoproterenol-induced ICl with a half-maximally effective concentration of approximately 1 nM. ET-1 also inhibited the forskolin-induced current in a similar concentration range. The effects of ET-1 were abolished by pre-treatment of the cells with pertussis toxin. Since ET-1 was ineffective at inhibiting the ICl induced by internal dialysis with cyclic AMP, it is unlikely that the Gi-protein had a direct effect on channel gating or phosphorylation of the channel by PKA. It is concluded that ET-1 inhibited the cardiac PKA-dependent ICl by attenuating activation of adenylate cyclase and that this effect was mediated by a pertussis toxin-sensitive G-protein, presumably Gi.

Original languageEnglish (US)
Pages (from-to)S227-230
JournalThe Japanese journal of physiology
Volume44 Suppl 2
StatePublished - 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology


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