The effects of local vanadium treatment on angiogenesis and chondrogenesis during fracture healing

David N. Paglia, Aaron Wey, Andrew G. Park, Eric A. Breitbart, Siddhant K. Mehta, John D. Bogden, Francis W. Kemp, Joseph Benevenia, J. Patrick O'Connor, Sheldon S. Lin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study quantified the effects of local intramedullary delivery of an organic vanadium salt, which may act as an insulin-mimetic on fracture healing. Using a BB Wistar rat femoral fracture model, local vanadyl acetylacetonate (VAC) was delivered to the fracture site and histomorphometry, mechanical testing, and immunohistochemistry were performed. Callus percent cartilage was 200% higher at day 7 (p < 0.05) and 88% higher at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Callus percent mineralized tissue was 37% higher at day 14 (p < 0.05) and 31% higher at day 21 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Maximum torque to failure was 104% and 154% higher at 4 weeks post-fracture (p < 0.05) for the healing femurs from the VAC-treated (1.5 and 3.0 mg/kg) animals. Animals treated with other VAC doses demonstrated increased mechanical parameters at 4 weeks (p < 0.05). Immunohistochemistry detected 62% more proliferating cells at days 7 (p < 0.05) and 94% more at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg VAC. Results showed 100% more vascular endothelial growth factor-C (VEGF-C) positive cells and 80% more blood vessels at day 7 (p < 0.05) within the callus subperiosteal region of VAC-treated animals (1.5 mg/kg) compared to controls. The results suggest that local VAC treatment affects chondrogenesis and angiogenesis within the first 7-10 days post-fracture, which leads to enhanced mineralized tissue formation and accelerated fracture repair as early as 3-4 weeks post-fracture.

Original languageEnglish (US)
Pages (from-to)1971-1978
Number of pages8
JournalJournal of Orthopaedic Research
Volume30
Issue number12
DOIs
StatePublished - Dec 1 2012

Fingerprint

Chondrogenesis
Vanadium
Fracture Healing
Bony Callus
Therapeutics
Immunohistochemistry
Inbred BB Rats
Vascular Endothelial Growth Factor C
Femoral Fractures
acetyl acetonate
Torque
Femur
Cartilage
Blood Vessels
Salts
Insulin

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Keywords

  • BB Wistar rat
  • bone regeneration
  • fracture healing
  • vanadium

Cite this

Paglia, David N. ; Wey, Aaron ; Park, Andrew G. ; Breitbart, Eric A. ; Mehta, Siddhant K. ; Bogden, John D. ; Kemp, Francis W. ; Benevenia, Joseph ; O'Connor, J. Patrick ; Lin, Sheldon S. / The effects of local vanadium treatment on angiogenesis and chondrogenesis during fracture healing. In: Journal of Orthopaedic Research. 2012 ; Vol. 30, No. 12. pp. 1971-1978.
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abstract = "This study quantified the effects of local intramedullary delivery of an organic vanadium salt, which may act as an insulin-mimetic on fracture healing. Using a BB Wistar rat femoral fracture model, local vanadyl acetylacetonate (VAC) was delivered to the fracture site and histomorphometry, mechanical testing, and immunohistochemistry were performed. Callus percent cartilage was 200{\%} higher at day 7 (p < 0.05) and 88{\%} higher at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Callus percent mineralized tissue was 37{\%} higher at day 14 (p < 0.05) and 31{\%} higher at day 21 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Maximum torque to failure was 104{\%} and 154{\%} higher at 4 weeks post-fracture (p < 0.05) for the healing femurs from the VAC-treated (1.5 and 3.0 mg/kg) animals. Animals treated with other VAC doses demonstrated increased mechanical parameters at 4 weeks (p < 0.05). Immunohistochemistry detected 62{\%} more proliferating cells at days 7 (p < 0.05) and 94{\%} more at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg VAC. Results showed 100{\%} more vascular endothelial growth factor-C (VEGF-C) positive cells and 80{\%} more blood vessels at day 7 (p < 0.05) within the callus subperiosteal region of VAC-treated animals (1.5 mg/kg) compared to controls. The results suggest that local VAC treatment affects chondrogenesis and angiogenesis within the first 7-10 days post-fracture, which leads to enhanced mineralized tissue formation and accelerated fracture repair as early as 3-4 weeks post-fracture.",
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The effects of local vanadium treatment on angiogenesis and chondrogenesis during fracture healing. / Paglia, David N.; Wey, Aaron; Park, Andrew G.; Breitbart, Eric A.; Mehta, Siddhant K.; Bogden, John D.; Kemp, Francis W.; Benevenia, Joseph; O'Connor, J. Patrick; Lin, Sheldon S.

In: Journal of Orthopaedic Research, Vol. 30, No. 12, 01.12.2012, p. 1971-1978.

Research output: Contribution to journalArticle

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T1 - The effects of local vanadium treatment on angiogenesis and chondrogenesis during fracture healing

AU - Paglia, David N.

AU - Wey, Aaron

AU - Park, Andrew G.

AU - Breitbart, Eric A.

AU - Mehta, Siddhant K.

AU - Bogden, John D.

AU - Kemp, Francis W.

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AU - Lin, Sheldon S.

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AB - This study quantified the effects of local intramedullary delivery of an organic vanadium salt, which may act as an insulin-mimetic on fracture healing. Using a BB Wistar rat femoral fracture model, local vanadyl acetylacetonate (VAC) was delivered to the fracture site and histomorphometry, mechanical testing, and immunohistochemistry were performed. Callus percent cartilage was 200% higher at day 7 (p < 0.05) and 88% higher at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Callus percent mineralized tissue was 37% higher at day 14 (p < 0.05) and 31% higher at day 21 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Maximum torque to failure was 104% and 154% higher at 4 weeks post-fracture (p < 0.05) for the healing femurs from the VAC-treated (1.5 and 3.0 mg/kg) animals. Animals treated with other VAC doses demonstrated increased mechanical parameters at 4 weeks (p < 0.05). Immunohistochemistry detected 62% more proliferating cells at days 7 (p < 0.05) and 94% more at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg VAC. Results showed 100% more vascular endothelial growth factor-C (VEGF-C) positive cells and 80% more blood vessels at day 7 (p < 0.05) within the callus subperiosteal region of VAC-treated animals (1.5 mg/kg) compared to controls. The results suggest that local VAC treatment affects chondrogenesis and angiogenesis within the first 7-10 days post-fracture, which leads to enhanced mineralized tissue formation and accelerated fracture repair as early as 3-4 weeks post-fracture.

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