The effects of local vanadium treatment on angiogenesis and chondrogenesis during fracture healing

David N. Paglia, Aaron Wey, Andrew G. Park, Eric A. Breitbart, Siddhant K. Mehta, John D. Bogden, Francis W. Kemp, Joseph Benevenia, J. Patrick O'Connor, Sheldon S. Lin

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Abstract

This study quantified the effects of local intramedullary delivery of an organic vanadium salt, which may act as an insulin-mimetic on fracture healing. Using a BB Wistar rat femoral fracture model, local vanadyl acetylacetonate (VAC) was delivered to the fracture site and histomorphometry, mechanical testing, and immunohistochemistry were performed. Callus percent cartilage was 200% higher at day 7 (p < 0.05) and 88% higher at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Callus percent mineralized tissue was 37% higher at day 14 (p < 0.05) and 31% higher at day 21 (p < 0.05) in the animals treated with 1.5 mg/kg of VAC. Maximum torque to failure was 104% and 154% higher at 4 weeks post-fracture (p < 0.05) for the healing femurs from the VAC-treated (1.5 and 3.0 mg/kg) animals. Animals treated with other VAC doses demonstrated increased mechanical parameters at 4 weeks (p < 0.05). Immunohistochemistry detected 62% more proliferating cells at days 7 (p < 0.05) and 94% more at day 10 (p < 0.05) in the animals treated with 1.5 mg/kg VAC. Results showed 100% more vascular endothelial growth factor-C (VEGF-C) positive cells and 80% more blood vessels at day 7 (p < 0.05) within the callus subperiosteal region of VAC-treated animals (1.5 mg/kg) compared to controls. The results suggest that local VAC treatment affects chondrogenesis and angiogenesis within the first 7-10 days post-fracture, which leads to enhanced mineralized tissue formation and accelerated fracture repair as early as 3-4 weeks post-fracture.

Original languageEnglish (US)
Pages (from-to)1971-1978
Number of pages8
JournalJournal of Orthopaedic Research
Volume30
Issue number12
DOIs
StatePublished - Dec 1 2012

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All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine

Keywords

  • BB Wistar rat
  • bone regeneration
  • fracture healing
  • vanadium

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