The effects of organic nitrogen sources on growth of cell cultures of Douglas‐fir

Edward Kirby

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In order to establish rapidly growing, friable cell cultures of Douglas‐fir (Pseudotsuga menziesii [Mirb. (Franco)], the effects of organic sources of nitrogen on growth were investigated. Of the nitrogen sources studied, including allantoin, allantoic acid, glutamine and glutamic acid, all were capable of increasing growth. Glutamine (50 mM) produced the most marked increase in growth boosting dry weight production to a level of four times that of controls. Glutamine additions also eliminated the lag phase of growth and caused cells to become densely cytoplasmic. Results are discussed in relation to the pathway for assimilation of nitrogen.

Original languageEnglish (US)
Pages (from-to)114-117
Number of pages4
JournalPhysiologia Plantarum
Volume56
Issue number1
DOIs
StatePublished - Jan 1 1982

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Keywords

  • Glutamine
  • gymnosperm
  • nitrogen assimilation
  • ureide

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