TY - JOUR
T1 - The evolution of drug resistance in Mycobacterium tuberculosis
T2 - From a mono-rifampin-resistant cluster into increasingly multidrug-resistant variants in an HIV-seropositive population
AU - Bifani, Pablo
AU - Mathema, Barun
AU - Kurepina, Natalia
AU - Shashkina, Elena
AU - Bertout, Julie
AU - Blanchis, Anne Sophie
AU - Moghazeh, Soraya
AU - Driscoll, Jeffrey
AU - Gicquel, Brigitte
AU - Frothingham, Richard
AU - Kreiswirth, Barry N.
N1 - Funding Information:
1Pasteur Institute, Brussels, Belgium; 2Public Health Research Institute Tuberculosis Center, Newark, New Jersey; 3Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, and 4Wadsworth Center, New York State Department of Health, Albany; 5Durham Veterans Affairs Medical Center, Durham, North Carolina; 6Institut National de la Santé et de la Recherche Médicale U-629, Institut Pasteur de Lille, Lille, and 7Unité de Génétique Mycobactérienne, Institut Pasteur, Paris, France
Funding Information:
Received 8 October 2007; accepted 16 January 2008; electronically published 22 May 2008. Potential conflicts of interest: none reported. Presented in part: International Conference on Emerging Infectious Diseases, Atlanta, July 2000 (oral presentation). Financial support: Centers for Disease Control and Prevention, Tuberculosis Surveillance Network; Public Health Research Institute. Reprints or correspondence: P. Bifani, Pasteur Institute, Engeland Rd. 642, B-1180, Brussels, Belgium (pbifani@pasteur.be); or, B. Kreiswirth, Public Health Research Institute Tuberculosis Center, 225 Warren St., Newark, NJ 07103, USA (barry@umndj.org).
PY - 2008/7/1
Y1 - 2008/7/1
N2 - We describe the genotypic and phenotypic characteristics of a mono-rifampin-resistant (RIFR) Mycobacterium tuberculosis strain cluster (designated AU-RIFR) and the acquisition of additional drug resistance. Drug susceptibility, sequences of regions that determine drug resistance, and basic clinical data were examined. A rare codon duplication (514TTC) in rpoB conferring high levels of RIFR (minimum inhibitory concentration of >256 μg/mL) in 29 isolates was identified. AU-RIFR strains developed secondary resistance to isoniazid and 7 resistance combinations to 6 different antibiotics. Patients infected with AU-RIFR strains were primarily immunocompromised. These data suggest that host factors, such as HIV status, may allow dissemination of mono-RIF R strains and facilitate the accumulation of additional drug resistance.
AB - We describe the genotypic and phenotypic characteristics of a mono-rifampin-resistant (RIFR) Mycobacterium tuberculosis strain cluster (designated AU-RIFR) and the acquisition of additional drug resistance. Drug susceptibility, sequences of regions that determine drug resistance, and basic clinical data were examined. A rare codon duplication (514TTC) in rpoB conferring high levels of RIFR (minimum inhibitory concentration of >256 μg/mL) in 29 isolates was identified. AU-RIFR strains developed secondary resistance to isoniazid and 7 resistance combinations to 6 different antibiotics. Patients infected with AU-RIFR strains were primarily immunocompromised. These data suggest that host factors, such as HIV status, may allow dissemination of mono-RIF R strains and facilitate the accumulation of additional drug resistance.
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U2 - 10.1086/588822
DO - 10.1086/588822
M3 - Article
C2 - 18498237
AN - SCOPUS:46349091974
VL - 198
SP - 90
EP - 94
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 1
ER -