The expression profile of microRNAs in mouse embryos

Junichi Mineno, Sachiko Okamoto, Tatsuya Ando, Masahiro Sato, Hideto Chono, Hiroyuki Izu, Masanori Takayama, Kiyozo Asada, Oleg Mirochnitchenko, Masayori Inouye, Ikunoshin Kato

Research output: Contribution to journalArticlepeer-review

206 Scopus citations


MicroRNAs (miRNAs), which are non-coding RNAs 18-25 nt in length, regulate a variety of biological processes, including vertebrate development. To identify new species of miRNA and to simultaneously obtain a comprehensive quantitative profile of small RNA expression in mouse embryos, we used the massively parallel signature sequencing technology that potentially identifies virtually all of the small RNAs in a sample. This approach allowed us to detect a total of 390 miRNAs, including 195 known miRNAs covering ∼80% of previously registered mouse miRNAs as well as 195 new miRNAs, which are so far unknown in mouse. Some of these miRNAs showed temporal expression profiles during prenatal development (E9.5, E10.5 and E11.5). Several miRNAs were positioned in polycistron clusters, including one particular large transcription unit consisting of 16 known and 23 new miRNAs. Our results indicate existence of a significant number of new miRNAs expressed at specific stages of mammalian embryonic development and which were not detected by earlier methods.

Original languageEnglish (US)
Pages (from-to)1765-1771
Number of pages7
JournalNucleic acids research
Issue number6
StatePublished - 2006

All Science Journal Classification (ASJC) codes

  • Genetics

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