The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase

Jae H. Choi, Paula G. Bertram, Ryan Drenan, John Carvalho, Heather H. Zhou, X. F.Steven Zheng

Research output: Contribution to journalArticle

79 Scopus citations

Abstract

CLIP-170/Restin belongs to a family of conserved microtubule (MT)-associated proteins, which are important for MT organization and functions. CLIP-170 is a phosphoprotein and phosphorylation is thought to regulate the binding of CLIP-170 to MTs. However, little is known about the kinase(s) involved. In this study, we show that FKBP12-rapamycin-associated protein (FRAP, also called mTOR/RAFT) interacts with CLIP-170. CLIP-170 is phosphorylated in vivo at multiple sites, including rapamycin-sensitive and -insensitive sites, and is phosphorylated by FRAP in vitro at the rapamycin-sensitive sites. In addition, rapamycin inhibited the ability of CLIP-170 to bind to MTs. Our observations suggest that multiple CLIP-170 kinases are involved in positive and negative control of CLIP-170, and FRAP is a CLIP-170 kinase positively regulating the MT-binding behavior of CLIP-170.

Original languageEnglish (US)
Pages (from-to)988-994
Number of pages7
JournalEMBO Reports
Volume3
Issue number10
DOIs
StatePublished - Oct 1 2002

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

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    Choi, J. H., Bertram, P. G., Drenan, R., Carvalho, J., Zhou, H. H., & Zheng, X. F. S. (2002). The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase. EMBO Reports, 3(10), 988-994. https://doi.org/10.1093/embo-reports/kvf197