Abstract
The aim of this study was to identify the food resources of Heligmosomoides polygyrus, a gastrointestinal nematode of mice. Gastrointestinal nematodes obtain food from 1 of 3 compartments: host ingesta, blood, or intestinal tissue. A method was developed to label these compartments differentially in the living host using 2 fluorescent marker dyes and to record in situ feeding activity of the parasite. Fluoresbrite is a yellow-green fluorescent dye bound to small-diameter beads that are membrane impermeable. Thus, it is restricted to the bloodstream when introduced there, or it remains in the ingesta when fed to the host. Rhodamine B, a red fluorescent dye, is membrane permeable and stains tissue cytoplasm. These dyes were fed to or injected into the bloodstream of the host. Following treatment, the worms were removed, and the contents of the worm intestines were examined by fluorescent microscopy. Worm intestinal contents only fluoresced with rhodamine B dye. These results suggest that H. polygyrus adults feed on tissue in the living host and not on host ingesta or blood.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 24-28 |
| Number of pages | 5 |
| Journal | Journal of Parasitology |
| Volume | 80 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1994 |
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All Science Journal Classification (ASJC) codes
- Parasitology
- Ecology, Evolution, Behavior and Systematics
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The food resource of adult Heligmosomoides polygyrus in the small intestine. / Bansemir, A. D.; Sukhdeo, M. V.K.
In: Journal of Parasitology, Vol. 80, No. 1, 01.01.1994, p. 24-28.Research output: Contribution to journal › Article
TY - JOUR
T1 - The food resource of adult Heligmosomoides polygyrus in the small intestine
AU - Bansemir, A. D.
AU - Sukhdeo, M. V.K.
PY - 1994/1/1
Y1 - 1994/1/1
N2 - The aim of this study was to identify the food resources of Heligmosomoides polygyrus, a gastrointestinal nematode of mice. Gastrointestinal nematodes obtain food from 1 of 3 compartments: host ingesta, blood, or intestinal tissue. A method was developed to label these compartments differentially in the living host using 2 fluorescent marker dyes and to record in situ feeding activity of the parasite. Fluoresbrite is a yellow-green fluorescent dye bound to small-diameter beads that are membrane impermeable. Thus, it is restricted to the bloodstream when introduced there, or it remains in the ingesta when fed to the host. Rhodamine B, a red fluorescent dye, is membrane permeable and stains tissue cytoplasm. These dyes were fed to or injected into the bloodstream of the host. Following treatment, the worms were removed, and the contents of the worm intestines were examined by fluorescent microscopy. Worm intestinal contents only fluoresced with rhodamine B dye. These results suggest that H. polygyrus adults feed on tissue in the living host and not on host ingesta or blood.
AB - The aim of this study was to identify the food resources of Heligmosomoides polygyrus, a gastrointestinal nematode of mice. Gastrointestinal nematodes obtain food from 1 of 3 compartments: host ingesta, blood, or intestinal tissue. A method was developed to label these compartments differentially in the living host using 2 fluorescent marker dyes and to record in situ feeding activity of the parasite. Fluoresbrite is a yellow-green fluorescent dye bound to small-diameter beads that are membrane impermeable. Thus, it is restricted to the bloodstream when introduced there, or it remains in the ingesta when fed to the host. Rhodamine B, a red fluorescent dye, is membrane permeable and stains tissue cytoplasm. These dyes were fed to or injected into the bloodstream of the host. Following treatment, the worms were removed, and the contents of the worm intestines were examined by fluorescent microscopy. Worm intestinal contents only fluoresced with rhodamine B dye. These results suggest that H. polygyrus adults feed on tissue in the living host and not on host ingesta or blood.
UR - http://www.scopus.com/inward/record.url?scp=0028063868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028063868&partnerID=8YFLogxK
U2 - 10.2307/3283340
DO - 10.2307/3283340
M3 - Article
C2 - 8308654
AN - SCOPUS:0028063868
VL - 80
SP - 24
EP - 28
JO - Journal of Parasitology
JF - Journal of Parasitology
SN - 0022-3395
IS - 1
ER -