The food resource of adult Heligmosomoides polygyrus in the small intestine

A. D. Bansemir, M. V.K. Sukhdeo

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The aim of this study was to identify the food resources of Heligmosomoides polygyrus, a gastrointestinal nematode of mice. Gastrointestinal nematodes obtain food from 1 of 3 compartments: host ingesta, blood, or intestinal tissue. A method was developed to label these compartments differentially in the living host using 2 fluorescent marker dyes and to record in situ feeding activity of the parasite. Fluoresbrite is a yellow-green fluorescent dye bound to small-diameter beads that are membrane impermeable. Thus, it is restricted to the bloodstream when introduced there, or it remains in the ingesta when fed to the host. Rhodamine B, a red fluorescent dye, is membrane permeable and stains tissue cytoplasm. These dyes were fed to or injected into the bloodstream of the host. Following treatment, the worms were removed, and the contents of the worm intestines were examined by fluorescent microscopy. Worm intestinal contents only fluoresced with rhodamine B dye. These results suggest that H. polygyrus adults feed on tissue in the living host and not on host ingesta or blood.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalJournal of Parasitology
Volume80
Issue number1
DOIs
StatePublished - Jan 1 1994

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rhodamine B
Nematospiroides dubius
Heligmosomoides polygyrus
Fluorescent Dyes
Small Intestine
small intestine
Coloring Agents
dye
Food
dyes
food
resource
Gastrointestinal Contents
Membranes
fluorescent dyes
gastrointestinal nematodes
blood flow
Intestines
nematode
Microscopy

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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abstract = "The aim of this study was to identify the food resources of Heligmosomoides polygyrus, a gastrointestinal nematode of mice. Gastrointestinal nematodes obtain food from 1 of 3 compartments: host ingesta, blood, or intestinal tissue. A method was developed to label these compartments differentially in the living host using 2 fluorescent marker dyes and to record in situ feeding activity of the parasite. Fluoresbrite is a yellow-green fluorescent dye bound to small-diameter beads that are membrane impermeable. Thus, it is restricted to the bloodstream when introduced there, or it remains in the ingesta when fed to the host. Rhodamine B, a red fluorescent dye, is membrane permeable and stains tissue cytoplasm. These dyes were fed to or injected into the bloodstream of the host. Following treatment, the worms were removed, and the contents of the worm intestines were examined by fluorescent microscopy. Worm intestinal contents only fluoresced with rhodamine B dye. These results suggest that H. polygyrus adults feed on tissue in the living host and not on host ingesta or blood.",
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The food resource of adult Heligmosomoides polygyrus in the small intestine. / Bansemir, A. D.; Sukhdeo, M. V.K.

In: Journal of Parasitology, Vol. 80, No. 1, 01.01.1994, p. 24-28.

Research output: Contribution to journalArticle

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