The genome sequence of Desulfatibacillum alkenivorans AK-01: A blueprint for anaerobic alkane oxidation

A. V. Callaghan, B. E.L. Morris, I. A.C. Pereira, M. J. McInerney, R. N. Austin, J. T. Groves, Jerome Kukor, J. M. Suflita, Lily Young, Gerben Zylstra, B. Wawrik

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Desulfatibacillum alkenivorans AK-01 serves as a model organism for anaerobic alkane biodegradation because of its distinctive biochemistry and metabolic versatility. The D. alkenivorans genome provides a blueprint for understanding the genetic systems involved in alkane metabolism including substrate activation, CoA ligation, carbon-skeleton rearrangement and decarboxylation. Genomic analysis suggested a route to regenerate the fumarate needed for alkane activation via methylmalonyl-CoA and predicted the capability for syntrophic alkane metabolism, which was experimentally verified. Pathways involved in the oxidation of alkanes, alcohols, organic acids and n-saturated fatty acids coupled to sulfate reduction and the ability to grow chemolithoautotrophically were predicted. A complement of genes for motility and oxygen detoxification suggests that D. alkenivorans may be physiologically adapted to a wide range of environmental conditions. The D. alkenivorans genome serves as a platform for further study of anaerobic, hydrocarbon-oxidizing microorganisms and their roles in bioremediation, energy recovery and global carbon cycling.

Original languageEnglish (US)
Pages (from-to)101-113
Number of pages13
JournalEnvironmental microbiology
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Alkanes
alkane
alkanes
genome
Genome
oxidation
Carbon
metabolism
energy recovery
decarboxylation
Fumarates
Decarboxylation
Environmental Biodegradation
carbon
biochemistry
bioremediation
motility
Coenzyme A
Hydrocarbons
detoxification

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Callaghan, A. V., Morris, B. E. L., Pereira, I. A. C., McInerney, M. J., Austin, R. N., Groves, J. T., ... Wawrik, B. (2012). The genome sequence of Desulfatibacillum alkenivorans AK-01: A blueprint for anaerobic alkane oxidation. Environmental microbiology, 14(1), 101-113. https://doi.org/10.1111/j.1462-2920.2011.02516.x
Callaghan, A. V. ; Morris, B. E.L. ; Pereira, I. A.C. ; McInerney, M. J. ; Austin, R. N. ; Groves, J. T. ; Kukor, Jerome ; Suflita, J. M. ; Young, Lily ; Zylstra, Gerben ; Wawrik, B. / The genome sequence of Desulfatibacillum alkenivorans AK-01 : A blueprint for anaerobic alkane oxidation. In: Environmental microbiology. 2012 ; Vol. 14, No. 1. pp. 101-113.
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Callaghan, AV, Morris, BEL, Pereira, IAC, McInerney, MJ, Austin, RN, Groves, JT, Kukor, J, Suflita, JM, Young, L, Zylstra, G & Wawrik, B 2012, 'The genome sequence of Desulfatibacillum alkenivorans AK-01: A blueprint for anaerobic alkane oxidation', Environmental microbiology, vol. 14, no. 1, pp. 101-113. https://doi.org/10.1111/j.1462-2920.2011.02516.x

The genome sequence of Desulfatibacillum alkenivorans AK-01 : A blueprint for anaerobic alkane oxidation. / Callaghan, A. V.; Morris, B. E.L.; Pereira, I. A.C.; McInerney, M. J.; Austin, R. N.; Groves, J. T.; Kukor, Jerome; Suflita, J. M.; Young, Lily; Zylstra, Gerben; Wawrik, B.

In: Environmental microbiology, Vol. 14, No. 1, 01.01.2012, p. 101-113.

Research output: Contribution to journalArticle

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AU - Groves, J. T.

AU - Kukor, Jerome

AU - Suflita, J. M.

AU - Young, Lily

AU - Zylstra, Gerben

AU - Wawrik, B.

PY - 2012/1/1

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N2 - Desulfatibacillum alkenivorans AK-01 serves as a model organism for anaerobic alkane biodegradation because of its distinctive biochemistry and metabolic versatility. The D. alkenivorans genome provides a blueprint for understanding the genetic systems involved in alkane metabolism including substrate activation, CoA ligation, carbon-skeleton rearrangement and decarboxylation. Genomic analysis suggested a route to regenerate the fumarate needed for alkane activation via methylmalonyl-CoA and predicted the capability for syntrophic alkane metabolism, which was experimentally verified. Pathways involved in the oxidation of alkanes, alcohols, organic acids and n-saturated fatty acids coupled to sulfate reduction and the ability to grow chemolithoautotrophically were predicted. A complement of genes for motility and oxygen detoxification suggests that D. alkenivorans may be physiologically adapted to a wide range of environmental conditions. The D. alkenivorans genome serves as a platform for further study of anaerobic, hydrocarbon-oxidizing microorganisms and their roles in bioremediation, energy recovery and global carbon cycling.

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