The geochemical record of the ancient nitrogen cycle, nitrogen isotopes, and metal cofactors

Linda V. Godfrey, Jennifer B. Glass

Research output: Chapter in Book/Report/Conference proceedingChapter

28 Citations (Scopus)

Abstract

The nitrogen (N) cycle is the only global biogeochemical cycle that is driven by biological functions involving the interaction of many microorganisms. The N cycle has evolved over geological time and its interaction with the oxygen cycle has had profound effects on the evolution and timing of Earth's atmosphere oxygenation (Falkowski and Godfrey, 2008). Almost every enzyme that microorganisms use to manipulate N contains redox-sensitive metals. Bioavailability of these metals has changed through time as a function of varying redox conditions, and likely influenced the biological underpinnings of the N cycle. It is possible to construct a record through geological time using N isotopes and metal concentrations in sediments to determine when the different stages of the N cycle evolved and the role metal availability played in the development of key enzymes. The same techniques are applicable to understanding the operation and changes in the N cycle through geological time. However, N and many of the redox-sensitive metals in some of their oxidation states are mobile and the isotopic composition or distribution can be altered by subsequent processes leading to erroneous conclusions. This chapter reviews the enzymology and metal cofactors of the N cycle and describes proper utilization of methods used to reconstruct evolution of the N cycle through time.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages483-506
Number of pages24
EditionC
DOIs
StatePublished - Jan 1 2011

Publication series

NameMethods in Enzymology
NumberC
Volume486
ISSN (Print)0076-6879

Fingerprint

Nitrogen Isotopes
Nitrogen Cycle
Nitrogen
Metals
Oxidation-Reduction
Microorganisms
Earth atmosphere
Oxygenation
Enzymes
Atmosphere
Isotopes
Biological Availability
Sediments
Availability
Oxygen
Oxidation
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Godfrey, L. V., & Glass, J. B. (2011). The geochemical record of the ancient nitrogen cycle, nitrogen isotopes, and metal cofactors. In Methods in Enzymology (C ed., pp. 483-506). (Methods in Enzymology; Vol. 486, No. C). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-381294-0.00022-5
Godfrey, Linda V. ; Glass, Jennifer B. / The geochemical record of the ancient nitrogen cycle, nitrogen isotopes, and metal cofactors. Methods in Enzymology. C. ed. Academic Press Inc., 2011. pp. 483-506 (Methods in Enzymology; C).
@inbook{f7f98543a92047de94e450c6017fd7c4,
title = "The geochemical record of the ancient nitrogen cycle, nitrogen isotopes, and metal cofactors",
abstract = "The nitrogen (N) cycle is the only global biogeochemical cycle that is driven by biological functions involving the interaction of many microorganisms. The N cycle has evolved over geological time and its interaction with the oxygen cycle has had profound effects on the evolution and timing of Earth's atmosphere oxygenation (Falkowski and Godfrey, 2008). Almost every enzyme that microorganisms use to manipulate N contains redox-sensitive metals. Bioavailability of these metals has changed through time as a function of varying redox conditions, and likely influenced the biological underpinnings of the N cycle. It is possible to construct a record through geological time using N isotopes and metal concentrations in sediments to determine when the different stages of the N cycle evolved and the role metal availability played in the development of key enzymes. The same techniques are applicable to understanding the operation and changes in the N cycle through geological time. However, N and many of the redox-sensitive metals in some of their oxidation states are mobile and the isotopic composition or distribution can be altered by subsequent processes leading to erroneous conclusions. This chapter reviews the enzymology and metal cofactors of the N cycle and describes proper utilization of methods used to reconstruct evolution of the N cycle through time.",
author = "Godfrey, {Linda V.} and Glass, {Jennifer B.}",
year = "2011",
month = "1",
day = "1",
doi = "10.1016/B978-0-12-381294-0.00022-5",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
number = "C",
pages = "483--506",
booktitle = "Methods in Enzymology",
address = "United States",
edition = "C",

}

Godfrey, LV & Glass, JB 2011, The geochemical record of the ancient nitrogen cycle, nitrogen isotopes, and metal cofactors. in Methods in Enzymology. C edn, Methods in Enzymology, no. C, vol. 486, Academic Press Inc., pp. 483-506. https://doi.org/10.1016/B978-0-12-381294-0.00022-5

The geochemical record of the ancient nitrogen cycle, nitrogen isotopes, and metal cofactors. / Godfrey, Linda V.; Glass, Jennifer B.

Methods in Enzymology. C. ed. Academic Press Inc., 2011. p. 483-506 (Methods in Enzymology; Vol. 486, No. C).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - The geochemical record of the ancient nitrogen cycle, nitrogen isotopes, and metal cofactors

AU - Godfrey, Linda V.

AU - Glass, Jennifer B.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - The nitrogen (N) cycle is the only global biogeochemical cycle that is driven by biological functions involving the interaction of many microorganisms. The N cycle has evolved over geological time and its interaction with the oxygen cycle has had profound effects on the evolution and timing of Earth's atmosphere oxygenation (Falkowski and Godfrey, 2008). Almost every enzyme that microorganisms use to manipulate N contains redox-sensitive metals. Bioavailability of these metals has changed through time as a function of varying redox conditions, and likely influenced the biological underpinnings of the N cycle. It is possible to construct a record through geological time using N isotopes and metal concentrations in sediments to determine when the different stages of the N cycle evolved and the role metal availability played in the development of key enzymes. The same techniques are applicable to understanding the operation and changes in the N cycle through geological time. However, N and many of the redox-sensitive metals in some of their oxidation states are mobile and the isotopic composition or distribution can be altered by subsequent processes leading to erroneous conclusions. This chapter reviews the enzymology and metal cofactors of the N cycle and describes proper utilization of methods used to reconstruct evolution of the N cycle through time.

AB - The nitrogen (N) cycle is the only global biogeochemical cycle that is driven by biological functions involving the interaction of many microorganisms. The N cycle has evolved over geological time and its interaction with the oxygen cycle has had profound effects on the evolution and timing of Earth's atmosphere oxygenation (Falkowski and Godfrey, 2008). Almost every enzyme that microorganisms use to manipulate N contains redox-sensitive metals. Bioavailability of these metals has changed through time as a function of varying redox conditions, and likely influenced the biological underpinnings of the N cycle. It is possible to construct a record through geological time using N isotopes and metal concentrations in sediments to determine when the different stages of the N cycle evolved and the role metal availability played in the development of key enzymes. The same techniques are applicable to understanding the operation and changes in the N cycle through geological time. However, N and many of the redox-sensitive metals in some of their oxidation states are mobile and the isotopic composition or distribution can be altered by subsequent processes leading to erroneous conclusions. This chapter reviews the enzymology and metal cofactors of the N cycle and describes proper utilization of methods used to reconstruct evolution of the N cycle through time.

UR - http://www.scopus.com/inward/record.url?scp=78650927006&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650927006&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-381294-0.00022-5

DO - 10.1016/B978-0-12-381294-0.00022-5

M3 - Chapter

C2 - 21185450

AN - SCOPUS:78650927006

T3 - Methods in Enzymology

SP - 483

EP - 506

BT - Methods in Enzymology

PB - Academic Press Inc.

ER -

Godfrey LV, Glass JB. The geochemical record of the ancient nitrogen cycle, nitrogen isotopes, and metal cofactors. In Methods in Enzymology. C ed. Academic Press Inc. 2011. p. 483-506. (Methods in Enzymology; C). https://doi.org/10.1016/B978-0-12-381294-0.00022-5