TY - JOUR
T1 - The glycosphingolipids of rat sublingual and submaxillary glands
AU - Slomiany, Amalia
AU - Annese, Carlo
AU - Slomiany, Bronislaw L.
N1 - Funding Information:
The rat submaxillary and sublingual glands were generously supplied by Dr. J. Moschera. This research was supported by N.I.H. Grant AM 15565-04 from the National Institutes of Arthritis, Metabolism and Digestive Diseases.
PY - 1976/8/23
Y1 - 1976/8/23
N2 - 1. 1. Glycosphingolipids have been isolated from rat sublingual and submaxillary glands by the procedure involving lipid extraction, column fractionation and thin-layer chromatography. 2. 2. The major neutral glycosphingolipids in rat sublingual and submaxillary glands were monohexosylceramide, dihexosylceramide, tetrahexosylceramide and pentahexosylceramide. Both types of glands exhibited a low content of trihexosylceramide. The fucose-containing glycosphingolipids were not found. 3. 3. The acidic glycosphingolipids in rat sublingual and submaxillary glands were composed of monohexose sulfatide, dihexose sulfatide and monosialoand disialogangliosides of hematoside series. In addition, small quantities of gangliosides containing hexosamines were also present. 4. 4. The distribution of acidic and neutral glycosphingolipids was similar in the sublingual and submaxillary glands, except for the tetrahexosylceramide and sultatides. Sublingual glands contained 1.5 and 3.0 times as much tetrahexosylceramide and sulfatides, respectively, as did submaxillary glands. 5. 5. The glycosphingolipids of submaxillary and sublingual glands showed large similarity in fatty acid composition. The fatty acid composition of gangliosides resembled each other, but differed remarkably from those of sulfatides and neutral glycosphingolipids in the docosanoate content.
AB - 1. 1. Glycosphingolipids have been isolated from rat sublingual and submaxillary glands by the procedure involving lipid extraction, column fractionation and thin-layer chromatography. 2. 2. The major neutral glycosphingolipids in rat sublingual and submaxillary glands were monohexosylceramide, dihexosylceramide, tetrahexosylceramide and pentahexosylceramide. Both types of glands exhibited a low content of trihexosylceramide. The fucose-containing glycosphingolipids were not found. 3. 3. The acidic glycosphingolipids in rat sublingual and submaxillary glands were composed of monohexose sulfatide, dihexose sulfatide and monosialoand disialogangliosides of hematoside series. In addition, small quantities of gangliosides containing hexosamines were also present. 4. 4. The distribution of acidic and neutral glycosphingolipids was similar in the sublingual and submaxillary glands, except for the tetrahexosylceramide and sultatides. Sublingual glands contained 1.5 and 3.0 times as much tetrahexosylceramide and sulfatides, respectively, as did submaxillary glands. 5. 5. The glycosphingolipids of submaxillary and sublingual glands showed large similarity in fatty acid composition. The fatty acid composition of gangliosides resembled each other, but differed remarkably from those of sulfatides and neutral glycosphingolipids in the docosanoate content.
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U2 - 10.1016/0005-2760(76)90175-2
DO - 10.1016/0005-2760(76)90175-2
M3 - Article
C2 - 952992
AN - SCOPUS:0017067622
SN - 0005-2760
VL - 441
SP - 316
EP - 326
JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
IS - 2
ER -