TY - JOUR
T1 - The human U1 snRNP-Specific U1A protein inhibits polyadenylation of its own pre-mRNA
AU - Boelens, Wilbert C.
AU - Jansen, Eric J.R.
AU - van Venrooij, Walther J.
AU - Stripecke, Renata
AU - Mattaj, Iain W.
AU - Gunderson, Samuel I.
N1 - Funding Information:
W. C. B. and S. I. G. made equal contributions to this work. The work was supported by The Netherlands Foundation for Chemical Research with financial aid from The Netherlands Organization for Scientific Research (to W. C. B.) and by the European Molecular Biology Laboratory and the Alexander von Humboldt Fellowship Program (to S. I. G.). We thank S. Bienroth and W. Keller for adenovirus L3 substrates, M. Bennett and J. Craft for making the mouse UIA cDNA sequence available to us prior to publication, M. Hentze for help and supervision in the construction of the BG-derived cell lines, M. Treier for the CMV-]un plasmids, and J. Bernubs, M. Hentze, A. Jarmolowski, C. Kam-bath, J. Lewis, G. Pruijn, B. Seraphin, and D. Tollervey for their comments on the manuscript.
PY - 1993/3/26
Y1 - 1993/3/26
N2 - Human, mouse, and Xenopus mRNAs encoding the U1 snRNP-specific U1 A protein contain a conserved 47 nt region in their 3′ untranslated regions (UTRs). In vitro studies show that human U1A protein binds to two sites within the conserved region that resemble, in part, the previously characterized UlA-binding site on U1 snRNA. Overexpression of human U1A protein in mouse cells results in down-regulation of endogenous mouse UlA mRNA accumulation. In vitro and in vivo experiments demonstrate that excess UIA protein specifically inhibits polyadenylation of pre-mRNAs that contain the conserved 3′ UTR from human UlA mRNA. Thus, U1 A protein regulates the production of its own mRNA via a mechanism that involves pre-mRNA binding and inhibition of polyadenylation.
AB - Human, mouse, and Xenopus mRNAs encoding the U1 snRNP-specific U1 A protein contain a conserved 47 nt region in their 3′ untranslated regions (UTRs). In vitro studies show that human U1A protein binds to two sites within the conserved region that resemble, in part, the previously characterized UlA-binding site on U1 snRNA. Overexpression of human U1A protein in mouse cells results in down-regulation of endogenous mouse UlA mRNA accumulation. In vitro and in vivo experiments demonstrate that excess UIA protein specifically inhibits polyadenylation of pre-mRNAs that contain the conserved 3′ UTR from human UlA mRNA. Thus, U1 A protein regulates the production of its own mRNA via a mechanism that involves pre-mRNA binding and inhibition of polyadenylation.
UR - http://www.scopus.com/inward/record.url?scp=0027471377&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027471377&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(93)90577-D
DO - 10.1016/0092-8674(93)90577-D
M3 - Article
C2 - 8458082
AN - SCOPUS:0027471377
SN - 0092-8674
VL - 72
SP - 881
EP - 892
JO - Cell
JF - Cell
IS - 6
ER -