The isolation and characterization of RNA coded by the micF gene in Escherichia coli

Janet Andersen, Nicholas Delihas, Kazuhiro Ikenaka, Parnela J. Green, Ophry Pines, Orhan Ilercil, Masayori Inouye

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

A new species of micF RNA, which contains 93 nucleotidej (a 4.5S size), was isolated from Escherichia coli. The sequence of the 4.5S micF RNA corresponds to positions G82 through U174 of the micF gene. The 5′ terminal end of this smaller micF RNA is triphosphorylated signifying that it is a primary transcript. Its promoter region, which is situated within the greater micF structural gene, has been identified and characterized by lafii fusion analysis. A 6S micF RNA species, which has a base composition predicted for a transcript from the full length gene has also been detected; however, the 4.5S micF RNA is the predominant species. The work clearly shows by biochemical identification the presence of chromosomally encoded micF RNA.

Original languageEnglish (US)
Pages (from-to)2089-2101
Number of pages13
JournalNucleic acids research
Volume15
Issue number5
DOIs
StatePublished - Mar 15 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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