The Mcp element mediates stable long-range chromosome-chromosome interactions in Drosophila

Julio Vazquez, Martin Müller, Vincenzo Pirrotta, John W. Sedat

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

Chromosome organization inside the nucleus is not random but rather is determined by a variety of factors, including interactions between chromosomes and nuclear components such as the nuclear envelope or nuclear matrix. Such interactions may be critical for proper nuclear organization, chromosome partitioning during cell division, and gene regulation. An important, but poorly documented subset, includes interactions between specific chromosomal regions. Interactions of this type are thought to be involved in long-range promoter regulation by distant enhancers or locus control regions and may underlie phenomena such as transvection. Here, we used an in vivo microscopy assay based on Lac Repressor/operator recognition to show that Mcp, a polycomb response element from the Drosophila bithorax complex, is able to mediate physical interaction between remote chromosomal regions. These interactions are tissue specific, can take place between multiple Mcp elements, and seem to be stable once established. We speculate that this ability to interact may be part of the mechanism through which Mcp mediates its regulatory function in the bithorax complex.

Original languageEnglish (US)
Pages (from-to)2158-2165
Number of pages8
JournalMolecular biology of the cell
Volume17
Issue number5
DOIs
StatePublished - May 2006

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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