TY - JOUR
T1 - The morphogenesis of bacteriophage φ6
T2 - Particles formed by nonsense mutants
AU - Mindich, Leonard
AU - Sinclair, Jacqueline Franzese
AU - Cohen, Jeffrey
N1 - Funding Information:
This investigation was supported in part by Public Health Service Grants No. AI-09861 (from the National Institute of Allergy and Infectious Diseases) and No. GM-70474 (from the National Institute of General Medical Sciences) awarded to L.M. J.F.S. was supported by Public Health Service Training Grant No. GM-01290 from the National Institute of General Medical Sciences.
PY - 1976/11
Y1 - 1976/11
N2 - φ6 is a bacteriophage containing a genome of three pieces of double-stranded RNA and an envelope composed of lipids and proteins. The particles formed during infection of a nonsuppressor host by wild-type virus and six classes of nonsense mutants were investigated in this study. Mutants defective in the synthesis of P5 and P1l formed particles with RNA and lipid and all virion proteins but P5 and P11. Mutants defective in the synthesis of P3 made particles with lipid and RNA but also lacked P6. Particles formed by mutants defective in P6 were similar and lacked P3. These particles do not adsorb to host cells, indicating a role for P3 and P6 in attachment. Particles formed by a mutant defective in the synthesis of P9, P5, and P11 contained RNA but no lipid and also lacked P10, P3, and P6, the other membrane proteins. Particles formed by a mutant unable to form P12, a nonstructural protein, also lacked P9, P10, P3, P6, P5, and P11, and contained no lipid. We conclude that the core of the virion can be formed without the membranous exterior and that membrane protein P9 and the nonstructural protein P12 are necessary for the formation of the lipid-containing structure.
AB - φ6 is a bacteriophage containing a genome of three pieces of double-stranded RNA and an envelope composed of lipids and proteins. The particles formed during infection of a nonsuppressor host by wild-type virus and six classes of nonsense mutants were investigated in this study. Mutants defective in the synthesis of P5 and P1l formed particles with RNA and lipid and all virion proteins but P5 and P11. Mutants defective in the synthesis of P3 made particles with lipid and RNA but also lacked P6. Particles formed by mutants defective in P6 were similar and lacked P3. These particles do not adsorb to host cells, indicating a role for P3 and P6 in attachment. Particles formed by a mutant defective in the synthesis of P9, P5, and P11 contained RNA but no lipid and also lacked P10, P3, and P6, the other membrane proteins. Particles formed by a mutant unable to form P12, a nonstructural protein, also lacked P9, P10, P3, P6, P5, and P11, and contained no lipid. We conclude that the core of the virion can be formed without the membranous exterior and that membrane protein P9 and the nonstructural protein P12 are necessary for the formation of the lipid-containing structure.
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U2 - 10.1016/0042-6822(76)90021-0
DO - 10.1016/0042-6822(76)90021-0
M3 - Article
C2 - 982850
AN - SCOPUS:0017103791
SN - 0042-6822
VL - 75
SP - 224
EP - 231
JO - Virology
JF - Virology
IS - 1
ER -