The neuronal identifier element is a cis-acting positive regulator of gene expression

R. D. McKinnon, T. M. Shinnick, J. G. Sutcliffe

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

A middle-repetitive DNA element termed the identifier (ID) sequence, located in introns of postnatal-onset neuronal-specific genes, is transcribed in early postnatal rats by RNA polymerase III (Pol III) specifically in the brain. We show that these Pol III transcripts, although brain specific in vivo, are also expressed in immortalized rodent cell lines in culture. We demonstrate that the ID sequence can act as a positive regulator or enhancer of RNA polymerase II gene expression in cell lines that express these RNAs but not in primary cells in which Pol III transcription of the ID sequence is absent. Thus, ID elements may be positive regulators of postnatal-onset neuronal-specific gene expression.

Original languageEnglish (US)
Pages (from-to)3751-3755
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number11
DOIs
StatePublished - 1986

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'The neuronal identifier element is a cis-acting positive regulator of gene expression'. Together they form a unique fingerprint.

Cite this