The oncofetal gene Pem encodes a homeodomain and is regulated in primordial and pre-muscle stem cells

Anna W. Sasaki, Jess Doskow, Carol L. MacLeod, Melissa B. Rogers, Lorraine J. Gudas, Miles F. Wilkinson

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The oncofetal gene, Pem, is expressed in a stage specific manner during murine ontogeny. The carboxy terminal portion of the predicted Pem protein has significant similarity to homeodomains of the Drosophila prd family. The Pem gene is expressed in undifferentiated embryonal stem (ES) and embryonal carcinoma (EC) cell lines. Pem mRNA is induced 35-fold in ES cells differentiated in the absence of retinoic acid. Pem mRNA is increased in EC cells differentiated towards parietal or visceral endoderm, consistent with the abundant Pem expression in embryonic yolk sac. In 10T mesenchymal stem cells committed to muscle cell differentiation, Pem mRNA expression is dramatically increased. The elevation in Pem expression preceded the induction of the muscle master regulatory gene, myoD. We conclude that the Pem gene encodes a candidate transcription factor which is developmentally regulated.

Original languageEnglish (US)
Pages (from-to)155-164
Number of pages10
JournalMechanisms of Development
Volume34
Issue number2-3
DOIs
StatePublished - Jun 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

Keywords

  • Embryonal stem cell
  • Homeobox
  • Muscle development
  • Oncofetal

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