The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function

Vinoth Sittaramane, Xiufang Pan, Derrick M. Glasco, Peng Huang, Suman Gurung, Anagha Bock, Shike Li, Hui Wang, Koichi Kawakami, Michael Matise, Anand Chandrasekhar

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Vangl2, a core component of the Planar Cell Polarity pathway, is necessary for the caudal migration of Facial Branchiomotor (FBM) neurons in the vertebrate hindbrain. Studies in zebrafish suggest that vangl2 functions largely non-cell autonomously to regulate FBM neuron migration out of rhombomere 4 (r4), but the cell-type within which it acts is not known. Here, we demonstrate that vangl2 functions largely in floor plate cells to regulate caudal neuronal migration. Furthermore, FBM neurons fail to migrate caudally in the mouse Gli2 mutant that lacks the floor plate, suggesting an evolutionarily conserved role for this cell type in neuronal migration. Although hindbrain floor plate cilia are disorganized in vangl2 mutant embryos, cilia appear to be dispensable for neuronal migration. Notably, Vangl2 is enriched in the basolateral, but not apical, membranes of floor plate cells. Taken together, our data suggest strongly that Vangl2 regulates FBM neuron migration by acting in floor plate cells, independently of cilia function.

Original languageEnglish (US)
Pages (from-to)400-412
Number of pages13
JournalDevelopmental Biology
Volume382
Issue number2
DOIs
StatePublished - Oct 15 2013

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Sittaramane, V., Pan, X., Glasco, D. M., Huang, P., Gurung, S., Bock, A., Li, S., Wang, H., Kawakami, K., Matise, M., & Chandrasekhar, A. (2013). The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function. Developmental Biology, 382(2), 400-412. https://doi.org/10.1016/j.ydbio.2013.08.017