The plasma lipoproteins as risk factors: Comparison of electrophoretic and ultracentrifugation results

Stephen B. Hulley, George Rhoads

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Epidemiologic data from 1,859 men aged 45-65 were examined to explore which approach to analyzing plasma lipids and lipoproteins would be most useful for clinical medicine. In addition to analyzing fasting plasma lipids (cholesterol and triglyceride), we used two chemical techniques to measure the three major classes of lipoproteins: ultracentrifugation with heparin/manganese precipitation which yielded values for HDL-, LDL- and VLDL-cholesterol, and quantitative electrophoresis which yielded values for α-, β- and pre-β-lipoprotein. Both measures of the cholesterol-rich lipoprotein class (LDL-cholesterol and β-lipoprotein) were related to CHD prevalence, but neither appeared to be superior to total serum cholesterol in the strength-approximately twofold-of the association. Both measures of the protein-rich lipoprotein class (HDL-cholesterol and α-lipoprotein) showed a two-fold inverse association with CHD, although the latter did not attain statistical significance. No member of the triglyceride-rich class (pre-β-lipoprotein, VLDL-cholesterol, or serum triglyceride itself) was independently associated with CHD. We conclude that plasma total cholesterol remains a good single test for most clinical situations because of the prognostic information it contains, its widespread availability and its role in treatment decisions; also that either the plasma α-lipoprotein or HDL-cholesterol level can provide additional information on the likelihood of disease.

Original languageEnglish (US)
Pages (from-to)773-777
Number of pages5
JournalMetabolism
Volume31
Issue number8
DOIs
StatePublished - Jan 1 1982

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Ultracentrifugation
Lipoproteins
VLDL Cholesterol
Triglycerides
Cholesterol
LDL Cholesterol
HDL Cholesterol
Lipids
Clinical Medicine
HDL Lipoproteins
Manganese
Serum
LDL Lipoproteins
Heparin
Electrophoresis
Fasting
Proteins
lipoprotein cholesterol

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Hulley, Stephen B. ; Rhoads, George. / The plasma lipoproteins as risk factors : Comparison of electrophoretic and ultracentrifugation results. In: Metabolism. 1982 ; Vol. 31, No. 8. pp. 773-777.
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The plasma lipoproteins as risk factors : Comparison of electrophoretic and ultracentrifugation results. / Hulley, Stephen B.; Rhoads, George.

In: Metabolism, Vol. 31, No. 8, 01.01.1982, p. 773-777.

Research output: Contribution to journalArticle

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