Objective: To investigate the regulation of NK cells cytotoxicity by SNAP-23. Methods: The expression of SNAP-23 was silenced by specific shRNA plasmid transfection using nucleofector. LDH release assay and β-hexosaminidase release assay were respectively used to detect the change of NK92 cytotoxicity and exocytosis after suppression of SNAP-23. Results: Specific shRNA could suppress the expression of SNAP-23 in NK92 cells. After that, the cytotoxicity and exocytosis of NK92 cells decreased. Conclusion: SNAP-23 can regulate the cytotoxicity through modulating exocytosis of NK cells.
|Original language||English (US)|
|Number of pages||4|
|Journal||Chinese Journal of Microbiology and Immunology|
|State||Published - Feb 28 2006|
All Science Journal Classification (ASJC) codes
- NK cell