Abstract
Objective: To investigate the regulation of NK cells cytotoxicity by SNAP-23. Methods: The expression of SNAP-23 was silenced by specific shRNA plasmid transfection using nucleofector. LDH release assay and β-hexosaminidase release assay were respectively used to detect the change of NK92 cytotoxicity and exocytosis after suppression of SNAP-23. Results: Specific shRNA could suppress the expression of SNAP-23 in NK92 cells. After that, the cytotoxicity and exocytosis of NK92 cells decreased. Conclusion: SNAP-23 can regulate the cytotoxicity through modulating exocytosis of NK cells.
Original language | English (US) |
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Pages (from-to) | 97-100 |
Number of pages | 4 |
Journal | Chinese Journal of Microbiology and Immunology |
Volume | 26 |
Issue number | 2 |
State | Published - Feb 28 2006 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Microbiology
- Immunology
- Virology
Keywords
- Cytotoxicity
- Exocytosis
- NK cell
- SNAP-23
- shRNA