The relationship between fibroblast growth and the dynamic stiffnesses of a DNA crosslinked hydrogel

Frank X. Jiang, Bernard Yurke, Rene S. Schloss, Bonnie L. Firestein, Noshir A. Langrana

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


The microenvironment of cells is dynamic and undergoes remodeling with time. This is evident in development, aging, pathological processes, and at tissue-biomaterial interfaces. But in contrast, the majority of the biomimetic materials have static properties. Here, we show that a previously developed DNA crosslinked hydrogel circumvents the need of environmental factors and undergoes controlled stiffness change via DNA delivery, a feasible approach to initiate property changes in vivo, different from previous attempts. Two types of fibroblasts, L929 and GFP, were subject to the alterations in substrate rigidity presented in the hydrogels. Our results show that exogenous DNA does not cause appreciable cell shape change. Cells do respond to mechanical alterations as demonstrated in the cell projection area and polarity (e.g., Soft vs. Soft → Medium), and the responses vary depending on magnitude (e.g., Soft → Medium vs. Soft → Stiff) and range of stiffness changes (e.g., Soft → Medium vs. Medium → Stiff). The two types of fibroblasts share specific responses in common (e.g., Soft → Medium), while differ in others (e.g., Medium → Stiff). For each cell type, the projection area and polarity respond differently. This approach provides insight into pathology (e.g., cancer) and tissue functioning, and assists in designing biomaterials with controlled dynamic stiffness by choosing the range and magnitude of stiffness change. Crown

Original languageEnglish (US)
Pages (from-to)1199-1212
Number of pages14
Issue number6
StatePublished - Feb 2010

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials


  • Aspect ratio
  • Crosslinking density
  • DNA crosslinked hydrogels
  • Dynamic stiffness
  • FAK expression
  • Projection area


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