THE ROLE OF GLUTAMINE SYNTHETASE IN THE INCORPORATION OF AMMONIUM IN SKELETONEMA COSTATUM (BACILLARIOPHYCEAE)

Paul G. Falkowski; Richard B. Rivkin

doi:10.1111/j.1529-8817.1976.tb02871.x

THE ROLE OF GLUTAMINE SYNTHETASE IN THE INCORPORATION OF AMMONIUM IN SKELETONEMA COSTATUM (BACILLARIOPHYCEAE)

Paul G. Falkowski, Richard B. Rivkin

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36 Scopus citations

Abstract

The K_m for ammonia for glutamine synthetase and glutamate dehydrogenase was measured in enzyme extracts from Skeletonema costatum (Grev.) Cleve. At similar physiological pH and temperature the half‐saturation constant for glutamine synthetase was 29 μM, whereas for GDH it was 28mM. On the basis of relative enzymic activity, as well as substrate affinity, it is suggested that glutamine synthetase is the enzyme primarily responsible for the incorporation of ammonium into the amino acid pool, when extracellular nitrogen is at ecological concentrations.

Original language	English (US)
Pages (from-to)	448-450
Number of pages	3
Journal	Journal of Phycology
Volume	12
Issue number	4
DOIs	https://doi.org/10.1111/j.1529-8817.1976.tb02871.x
State	Published - Dec 1976
Externally published	Yes

All Science Journal Classification (ASJC) codes

Aquatic Science
Plant Science

Keywords

Skeletonema
ammonium
glutamate dehydrogenase
glutamate synthase
glutamine synthetase

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10.1111/j.1529-8817.1976.tb02871.x

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AB - The Km for ammonia for glutamine synthetase and glutamate dehydrogenase was measured in enzyme extracts from Skeletonema costatum (Grev.) Cleve. At similar physiological pH and temperature the half‐saturation constant for glutamine synthetase was 29 μM, whereas for GDH it was 28mM. On the basis of relative enzymic activity, as well as substrate affinity, it is suggested that glutamine synthetase is the enzyme primarily responsible for the incorporation of ammonium into the amino acid pool, when extracellular nitrogen is at ecological concentrations.

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KW - ammonium

KW - glutamate dehydrogenase

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THE ROLE OF GLUTAMINE SYNTHETASE IN THE INCORPORATION OF AMMONIUM IN SKELETONEMA COSTATUM (BACILLARIOPHYCEAE)

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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