The role of the guanine insertion enzyme in Q-biosynthesis in drosophila melanogaster

Randy D. Mckinnon, Michael A. Wosnick, Bradley N. White

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Drosophila tRNA can be guanylated by a crude enzyme from rabbit reticulocytes. Guanylating activity is also present in crude extracts of adult Drosophila A major product of this reaction as well as several minor ones were resolved by RPC-5 chromatography. The main substrate of both the Drosophila and rabbit reticulocyte enzymes was the non-Q-containing aspartic acid tRNA, tRNAAsp. The Q-lacking (y) forms of asparagine, histidine and tyrosine tRNAs were also substrates and gave rise to the minor products of the reaction. In contrast, the Q- or Q*-containing (δ) forms of these tRNAs appear not to be substrates. The evidence strongly suggests that the guanyating enzyme is involved in Q biosynthesis and would be better termed a guanine replacement or pre-Q insertion enzyme.

Original languageEnglish (US)
Pages (from-to)4865-4876
Number of pages12
JournalNucleic acids research
Volume5
Issue number12
DOIs
StatePublished - Dec 1978
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'The role of the guanine insertion enzyme in Q-biosynthesis in drosophila melanogaster'. Together they form a unique fingerprint.

Cite this