The semaphorin receptor PlexinA3 mediates neuronal apoptosis during dorsal root ganglia development

Ayal Ben-Zvi, Osnat Manor, Melitta Schachner, Avraham Yaron, Marc Tessier-Lavigne, Oded Behar

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Extensive neuronal cell death during development is believed to be due to a limiting supply of neurotrophic factors. In vitro studies suggest that axon guidance molecules directly regulate neuronal survival, raising the possibility that they play a direct role in neuronal cell death in vivo. However, guidance errors may also influence survival indirectly due to loss of target-derived neurotrophic support. The role of guidance molecules in neuronal death in vivo has thus been difficult to decipher. Semaphorin3A, a repulsive guidance cue for sensory neurons, can induce sensory neuron death in vitro. Null mice studies of the Semaphorin3A coreceptors showed that guidance activity is mediated by PlexinA4, but PlexinA3 partially compensates in PlexinA4-/- mice. Here we demonstrate that both Plexins contribute to Sema3A-induced cell death in vitro, albeit in a different hierarchy. PlexinA3 is absolutely required, while PlexinA4 makes a smaller contribution to cell death. We found that PlexinA3 -/- mice, which, unlike PlexinA4-/- mice, do not exhibit sensory axon patterning defects, show reduced neuronal apoptosis and an increased number of DRG neurons. Semaphorin3A involvement in neuronal death in vivo was demonstrated by a sensitization experiment using the proapoptotic effector Bax. Our results identify Plexins as mediators of Semaphorin-induced cell death in vitro, and provide the first evidence implicating Semaphorin/Plexin signaling in neuronal survival independent of its role in axon guidance. The results also support the idea that naturally occurring neuronal cell death reflects not only competition for target-derived trophic factors, but also the action of proapoptotic signaling via a Semaphorin/Plexin pathway.

Original languageEnglish (US)
Pages (from-to)12427-12432
Number of pages6
JournalJournal of Neuroscience
Volume28
Issue number47
DOIs
StatePublished - Nov 19 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Keywords

  • Apoptosis
  • Cell death
  • DRG neurons
  • Embryo
  • Sensory neurons
  • Survival

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