The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation

Peter Hajdukiewicz, Zora Svab, Pal Maliga

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617 Scopus citations


The new pPZP Agrobacterium binary vectors are versatile, relatively small, stable and are fully sequenced. The vectors utilize the pTiT37 T-DNA border regions, the pBR322 bom site for mobilization from Escherichia coli to Agrobacterium, and the ColE1 and pVS1 plasmid origins for replication in E. coli and in Agrobacterium, respectively. Bacterial marker genes in the vectors confer resistance to chloramphenicol (pPZP100 series) or spectinomycin (pPZP200 series), allowing their use in Agrobacterium strains with different drug resistance markers. Plant marker genes in the binary vectors confer resistance to kanamycin or to gentamycin, and are adjacent to the left border (LB) of the transferred region. A lacZ α-peptide, with the pUC18 multiple cloning site (MCS), lies between the plant marker gene and the right border (RB). Since the RB is transferred first, drug resistance is obtained only if the passenger gene is present in the transgenic plants.

Original languageEnglish (US)
Pages (from-to)989-994
Number of pages6
JournalPlant Molecular Biology
Issue number6
StatePublished - Sep 1994

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


  • Agrobacterium
  • binary vectors
  • gentamycin resistance
  • kanamycin resistance
  • tobacco


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