TY - JOUR
T1 - The specificity and orientation of a TCR to its peptide-MHC class II ligands
AU - Sant'Angelo, Derek B.
AU - Waterbury, Greg
AU - Preston-Hurlburt, Paula
AU - Yoon, Sangwook Tim
AU - Medzhitov, Ruslan
AU - Hong, Soon Cheol
AU - Janeway, Charles A.
N1 - Funding Information:
The authors want to thank D. Loh, J. Kaye, and T. Geiger for the donation of Cosmid and phage genomic clones, D. Butkus, C. Hughes, and R. Flavell for egg injections used to generate the TCR transgenic mice, P. Ranney for help with animal care, A. Barlow for technical assistance, and L. Denzin for valuable discussions and critical reading of the manuscript. The authors acknowledge K. McCarthy for excellent secretarial help. This work was supported in part by grant AI-14579 and by the Howard Hughes Medical Institute. Correspondence should be addressed to C. A. J.
PY - 1996/4
Y1 - 1996/4
N2 - A T cell-mediated immune response is mainly determined by the 3-5 aa residues that protrude upwards from a peptide bound to an MHC molecule. Alterations of these peptide residues can diminish, eliminate or radically alter the signal that the T cell receives through its T cell receptor (TCR). We have used peptide immunizations of normal mice and mice carrying α or β chain TCR transgenes to identify three distinct peptide contact points. One, near the carboxyl terminus of the peptide, involves the β chain CDR3 region; the second was centrally located and interacted with both the α and β chain CDR3 loops; the third was near the amino terminus of the peptide, and affected Vα gene usage, but not the structure of CDR3 of either TCR chain. Based on these results, we propose an orientation for the TCR of this cloned line and argue for its generality.
AB - A T cell-mediated immune response is mainly determined by the 3-5 aa residues that protrude upwards from a peptide bound to an MHC molecule. Alterations of these peptide residues can diminish, eliminate or radically alter the signal that the T cell receives through its T cell receptor (TCR). We have used peptide immunizations of normal mice and mice carrying α or β chain TCR transgenes to identify three distinct peptide contact points. One, near the carboxyl terminus of the peptide, involves the β chain CDR3 region; the second was centrally located and interacted with both the α and β chain CDR3 loops; the third was near the amino terminus of the peptide, and affected Vα gene usage, but not the structure of CDR3 of either TCR chain. Based on these results, we propose an orientation for the TCR of this cloned line and argue for its generality.
UR - https://www.scopus.com/pages/publications/0029932899
UR - https://www.scopus.com/pages/publications/0029932899#tab=citedBy
U2 - 10.1016/S1074-7613(00)80250-2
DO - 10.1016/S1074-7613(00)80250-2
M3 - Article
C2 - 8612131
AN - SCOPUS:0029932899
SN - 1074-7613
VL - 4
SP - 367
EP - 376
JO - Immunity
JF - Immunity
IS - 4
ER -