The stage specific toxicity of 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin in embryos of the japanese medaka (Oryzias latipes)

Joseph D. Wisk, Keith Cooper

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Embryos of the Japanese medaka were individually exposed to varying concentrations of [3H]2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) in a static, nonrenewal system. The EC50 with 95% confidence interval (C.I.) to prevent hatching was 14 (11–17) nanograms (ng) of TCDD equivalents per liter (L) of water (parts per trillion). The LC50 with 95% C.I. for survival to 3 d posthatch was 9 (6–12) ng TCDD equivalents/L. The EC50 with 95% C.I. for embryos with minor lesions and severe, life‐threatening lesions were 3.5 (1.3–5.7) ng TCDD equivalents/L and 14 (12.4–15.6) ng TCDD equivalents/L, respectively. In a separate experiment that was terminated prior to the embryos hatching or dying, the EC50 for lesions was calculated to be 2.2 (1.4–3.0) ng TCDD equivalents/L. Based on the amount of TCDD equivalents recovered from dechorionated embryos, the ED50 with 95% C.I. for lesions was calculated to be 0.24 picograms of TCDD equivalents per milligram of dechorionated embryo weight (parts per billion). When the embryos were exposed to [3H]TCDD within 1 to 2 h after fertilization, no concentration dependent increase in visible lesions was observed until after the formation of the liver rudiment (day 4 of development). By exposing Japanese medaka embryos to lethal concentrations of TCDD beginning on different days of embryonic development, it was demonstrated that the sensitive period for toxicity was during liver formation on day 4 or 5 of development. The sensitive period for development was not caused by differences in TCDD absorption across the chorion. When embryos were exposed to [3H]TCDD prior to, during or after liver formation, there was no statistical difference in the dose of TCDD equivalents that crossed the chorion and entered the yolk and embryo.

Original languageEnglish (US)
Pages (from-to)1159-1169
Number of pages11
JournalEnvironmental Toxicology and Chemistry
Volume9
Issue number9
DOIs
StatePublished - Jan 1 1990

Fingerprint

Oryzias
TCDD
Liver
Toxicity
embryo
Embryonic Structures
toxicity
lesion
confidence interval
Confidence Intervals
Chorion
Water
Polychlorinated Dibenzodioxins
hatching
Experiments
embryonic development
Fertilization
Embryonic Development

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis

Keywords

  • Embryo
  • Medaka
  • Stage
  • TCDD
  • Toxicity

Cite this

@article{1118eddffa4e40e6a9776c8d08581643,
title = "The stage specific toxicity of 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin in embryos of the japanese medaka (Oryzias latipes)",
abstract = "Embryos of the Japanese medaka were individually exposed to varying concentrations of [3H]2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) in a static, nonrenewal system. The EC50 with 95{\%} confidence interval (C.I.) to prevent hatching was 14 (11–17) nanograms (ng) of TCDD equivalents per liter (L) of water (parts per trillion). The LC50 with 95{\%} C.I. for survival to 3 d posthatch was 9 (6–12) ng TCDD equivalents/L. The EC50 with 95{\%} C.I. for embryos with minor lesions and severe, life‐threatening lesions were 3.5 (1.3–5.7) ng TCDD equivalents/L and 14 (12.4–15.6) ng TCDD equivalents/L, respectively. In a separate experiment that was terminated prior to the embryos hatching or dying, the EC50 for lesions was calculated to be 2.2 (1.4–3.0) ng TCDD equivalents/L. Based on the amount of TCDD equivalents recovered from dechorionated embryos, the ED50 with 95{\%} C.I. for lesions was calculated to be 0.24 picograms of TCDD equivalents per milligram of dechorionated embryo weight (parts per billion). When the embryos were exposed to [3H]TCDD within 1 to 2 h after fertilization, no concentration dependent increase in visible lesions was observed until after the formation of the liver rudiment (day 4 of development). By exposing Japanese medaka embryos to lethal concentrations of TCDD beginning on different days of embryonic development, it was demonstrated that the sensitive period for toxicity was during liver formation on day 4 or 5 of development. The sensitive period for development was not caused by differences in TCDD absorption across the chorion. When embryos were exposed to [3H]TCDD prior to, during or after liver formation, there was no statistical difference in the dose of TCDD equivalents that crossed the chorion and entered the yolk and embryo.",
keywords = "Embryo, Medaka, Stage, TCDD, Toxicity",
author = "Wisk, {Joseph D.} and Keith Cooper",
year = "1990",
month = "1",
day = "1",
doi = "10.1002/etc.5620090907",
language = "English (US)",
volume = "9",
pages = "1159--1169",
journal = "Environmental Toxicology and Chemistry",
issn = "0730-7268",
publisher = "John Wiley and Sons Ltd",
number = "9",

}

TY - JOUR

T1 - The stage specific toxicity of 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin in embryos of the japanese medaka (Oryzias latipes)

AU - Wisk, Joseph D.

AU - Cooper, Keith

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Embryos of the Japanese medaka were individually exposed to varying concentrations of [3H]2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) in a static, nonrenewal system. The EC50 with 95% confidence interval (C.I.) to prevent hatching was 14 (11–17) nanograms (ng) of TCDD equivalents per liter (L) of water (parts per trillion). The LC50 with 95% C.I. for survival to 3 d posthatch was 9 (6–12) ng TCDD equivalents/L. The EC50 with 95% C.I. for embryos with minor lesions and severe, life‐threatening lesions were 3.5 (1.3–5.7) ng TCDD equivalents/L and 14 (12.4–15.6) ng TCDD equivalents/L, respectively. In a separate experiment that was terminated prior to the embryos hatching or dying, the EC50 for lesions was calculated to be 2.2 (1.4–3.0) ng TCDD equivalents/L. Based on the amount of TCDD equivalents recovered from dechorionated embryos, the ED50 with 95% C.I. for lesions was calculated to be 0.24 picograms of TCDD equivalents per milligram of dechorionated embryo weight (parts per billion). When the embryos were exposed to [3H]TCDD within 1 to 2 h after fertilization, no concentration dependent increase in visible lesions was observed until after the formation of the liver rudiment (day 4 of development). By exposing Japanese medaka embryos to lethal concentrations of TCDD beginning on different days of embryonic development, it was demonstrated that the sensitive period for toxicity was during liver formation on day 4 or 5 of development. The sensitive period for development was not caused by differences in TCDD absorption across the chorion. When embryos were exposed to [3H]TCDD prior to, during or after liver formation, there was no statistical difference in the dose of TCDD equivalents that crossed the chorion and entered the yolk and embryo.

AB - Embryos of the Japanese medaka were individually exposed to varying concentrations of [3H]2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) in a static, nonrenewal system. The EC50 with 95% confidence interval (C.I.) to prevent hatching was 14 (11–17) nanograms (ng) of TCDD equivalents per liter (L) of water (parts per trillion). The LC50 with 95% C.I. for survival to 3 d posthatch was 9 (6–12) ng TCDD equivalents/L. The EC50 with 95% C.I. for embryos with minor lesions and severe, life‐threatening lesions were 3.5 (1.3–5.7) ng TCDD equivalents/L and 14 (12.4–15.6) ng TCDD equivalents/L, respectively. In a separate experiment that was terminated prior to the embryos hatching or dying, the EC50 for lesions was calculated to be 2.2 (1.4–3.0) ng TCDD equivalents/L. Based on the amount of TCDD equivalents recovered from dechorionated embryos, the ED50 with 95% C.I. for lesions was calculated to be 0.24 picograms of TCDD equivalents per milligram of dechorionated embryo weight (parts per billion). When the embryos were exposed to [3H]TCDD within 1 to 2 h after fertilization, no concentration dependent increase in visible lesions was observed until after the formation of the liver rudiment (day 4 of development). By exposing Japanese medaka embryos to lethal concentrations of TCDD beginning on different days of embryonic development, it was demonstrated that the sensitive period for toxicity was during liver formation on day 4 or 5 of development. The sensitive period for development was not caused by differences in TCDD absorption across the chorion. When embryos were exposed to [3H]TCDD prior to, during or after liver formation, there was no statistical difference in the dose of TCDD equivalents that crossed the chorion and entered the yolk and embryo.

KW - Embryo

KW - Medaka

KW - Stage

KW - TCDD

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=0025169422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025169422&partnerID=8YFLogxK

U2 - 10.1002/etc.5620090907

DO - 10.1002/etc.5620090907

M3 - Article

AN - SCOPUS:0025169422

VL - 9

SP - 1159

EP - 1169

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

IS - 9

ER -