TY - JOUR
T1 - The stress-activated c-Jun protein kinase (JNK) is stimulated by lipoxygenase pathway product 12-HETE in RIN m5F cells
AU - Bleich, David
AU - Chen, Songyuan
AU - Wen, Yeshao
AU - Nadler, Jerry L.
N1 - Funding Information:
This work was funded in part by the Ross Foundation and a Juvenile Diabetes Foundation Fellowship Award (S.C.). The authors wish
PY - 1997/1/13
Y1 - 1997/1/13
N2 - Cytokine induced pancreatic β-cell destruction seen in Type 1 diabetes and islet graft rejection involves multiple intracellular signaling pathways that directly or indirectly lead to inflammatory damage or programmed cell death. IL-1β has been shown to stimulate the 12-lipoxygenase pathway product 12-HETE, in RIN m5F cells; however, the precise role of 12-LO activation in mediating cytokine effects is not clear. Since the stress-activated protein kinase, JNK, has been linked to cytokine mediated inflammatory actions, we studied the effect of two LO products, 12-HETE and 15-HETE, on JNK activity. We demonstrate that 1 nM 12-HETE stimulates JNK activity, while 1 nM 15-HETE, the 15-lipoxygenase pathway product, does not. These results suggest 12-HETE is a novel upstream signal for IL-1β induced JNK activation in RIN m5F cells.
AB - Cytokine induced pancreatic β-cell destruction seen in Type 1 diabetes and islet graft rejection involves multiple intracellular signaling pathways that directly or indirectly lead to inflammatory damage or programmed cell death. IL-1β has been shown to stimulate the 12-lipoxygenase pathway product 12-HETE, in RIN m5F cells; however, the precise role of 12-LO activation in mediating cytokine effects is not clear. Since the stress-activated protein kinase, JNK, has been linked to cytokine mediated inflammatory actions, we studied the effect of two LO products, 12-HETE and 15-HETE, on JNK activity. We demonstrate that 1 nM 12-HETE stimulates JNK activity, while 1 nM 15-HETE, the 15-lipoxygenase pathway product, does not. These results suggest 12-HETE is a novel upstream signal for IL-1β induced JNK activation in RIN m5F cells.
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U2 - 10.1006/bbrc.1996.5981
DO - 10.1006/bbrc.1996.5981
M3 - Article
C2 - 9016800
AN - SCOPUS:0031566151
SN - 0006-291X
VL - 230
SP - 448
EP - 451
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -