LH, and FSH were measured by radioimmunoassays in hamsters injected SC with LHRH on each day of the estrous cycle. The ovulatory dose of LHRH required to reverse the phenobarbital-block in proestrous (Day 4) hamsters was 100 ng as a single injection or 50 ng as two injections 2 hr apart while a tenfold greater dose was required in Day 3 animals. Hamsters injected on Day 1 or 2 could not be induced to ovulate with up to a total of 4 μg LHRH. LHRH generally resulted in significant increases in LH and FSH on all days of the cycle including larger pre- to posttreatment rises on Days 2 and 3 than on Day 1 and, with smaller LHRH doses, potentiation of LH and FSH release on Days 3 and 4.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the Society for Experimental Biology and Medicine|
|State||Published - Jul 1978|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)