The tko locus, site of a behavioral mutation in D. melanogaster, codes for a protein homologous to prokaryotic ribosomal protein S12

Constance S. Royden, Vincenzo Pirrotta, Lily Y. Jan

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

The tko (technical knockout) mutation is one of a family of behavioral mutations that cause "bang sensitivity" in D. melanogaster. Using P-element-mediated transformation, we show that a 3.1 kb piece of genomic DNA complements tko. This fragment contains only one complete transcript, 0.68 kb in length. This transcript is abundantly expressed through all stages of the life cycle, and we have isolated cDNAs corresponding to this transcript. Their sequence implies a protein product composed of 140 amino acids, which exhibits considerable sequence similarity to ribosomal protein S12 from both Euglena gracilis chloroplasts and E. coli. We suggest that tko codes for a mitochondrial ribosomal protein and that the tko phenotype results from defective mitochondria.

Original languageEnglish (US)
Pages (from-to)165-173
Number of pages9
JournalCell
Volume51
Issue number2
DOIs
StatePublished - Oct 23 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'The tko locus, site of a behavioral mutation in D. melanogaster, codes for a protein homologous to prokaryotic ribosomal protein S12'. Together they form a unique fingerprint.

Cite this