TY - JOUR
T1 - The trophic role of oligodendrocytes in the basal forebrain
AU - Dai, Xudong
AU - Lercher, Lauren D.
AU - Clinton, Patricia M.
AU - Du, Yangzhou
AU - Livingston, Denise L.
AU - Vieira, Cristina
AU - Yang, Lu
AU - Shen, Michael M.
AU - Dreyfus, Cheryl F.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Traditionally, the primary function of oligodendrocytes (OLGs) in the CNS has been considered to be myelination. Here, we investigated whether OLGs may play a trophic role, particularly during development. Neurotrophin expression was assessed in postnatal day 7 basal forebrain (BF) OLGs, using in situ hybridization and detection of myelin basic protein. Nerve growth factor, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) mRNAs were revealed in OLGs in vivo and in culture. To determine whether OLGs support nearby neurons, we examined the influence of OLGs on BF cholinergic neurons. Neuronal function was enhanced by cocultured OLGs and OLG conditioned medium. Moreover, trophic effects of OLG conditioned medium were partially blocked by K252a, a trk tyrosine kinase inhibitor, and by neutralizing anti-BDNF or anti-NT-3 antisera, indicating that neurotrophins may mediate these effects, perhaps in concert with other signals. Our studies support a novel role for OLGs in providing local trophic support for neurons in the CNS.
AB - Traditionally, the primary function of oligodendrocytes (OLGs) in the CNS has been considered to be myelination. Here, we investigated whether OLGs may play a trophic role, particularly during development. Neurotrophin expression was assessed in postnatal day 7 basal forebrain (BF) OLGs, using in situ hybridization and detection of myelin basic protein. Nerve growth factor, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) mRNAs were revealed in OLGs in vivo and in culture. To determine whether OLGs support nearby neurons, we examined the influence of OLGs on BF cholinergic neurons. Neuronal function was enhanced by cocultured OLGs and OLG conditioned medium. Moreover, trophic effects of OLG conditioned medium were partially blocked by K252a, a trk tyrosine kinase inhibitor, and by neutralizing anti-BDNF or anti-NT-3 antisera, indicating that neurotrophins may mediate these effects, perhaps in concert with other signals. Our studies support a novel role for OLGs in providing local trophic support for neurons in the CNS.
KW - Basal forebrain
KW - BDNF
KW - NGF
KW - NT-3
KW - Oligodendrocytes
KW - Trophic role of oligodendrocytes on cholinergic neurons
UR - https://www.scopus.com/pages/publications/0038383706
UR - https://www.scopus.com/pages/publications/0038383706#tab=citedBy
U2 - 10.1523/jneurosci.23-13-05846.2003
DO - 10.1523/jneurosci.23-13-05846.2003
M3 - Article
C2 - 12843289
AN - SCOPUS:0038383706
SN - 0270-6474
VL - 23
SP - 5846
EP - 5853
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 13
ER -